Anti-ARF1 antibody [ARFS 3F1] (ab11038)
Key features and details
- Mouse monoclonal [ARFS 3F1] to ARF1
- Suitable for: IHC-P, ICC/IF, ELISA, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-ARF1 antibody [ARFS 3F1]
See all ARF1 primary antibodies -
Description
Mouse monoclonal [ARFS 3F1] to ARF1 -
Host species
Mouse -
Specificity
This antibody is specific to class I ARF. -
Tested applications
Suitable for: IHC-P, ICC/IF, ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
SNQLRNQ
, corresponding to C terminal amino acids 174-180 of Human ARF1. -
Positive control
- BALB/3T3 whole cell lysate (ab7901) can be used as a positive control in WB.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
ARFS 3F1 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2a -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab11038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/50. | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. |
Target
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Function
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. -
Sequence similarities
Belongs to the small GTPase superfamily. Arf family. -
Cellular localization
Golgi apparatus. Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 375 Human
- Omim: 103180 Human
- SwissProt: P84077 Human
- Unigene: 286221 Human
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Alternative names
- ADP Ribosylation Factor 1 antibody
- ADP-ribosylation factor 1 antibody
- ARF 1 antibody
see all
Images
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![ELISA - Anti-ARF1 antibody [ARFS 3F1] (ab11038)](https://www.abcam.com/ps/products/11/ab11038/Images/ab11038-18718-anti-arf1-antibody-arfs-3f1-elisa.jpg)
ELISA - Anti-ARF1 antibody [ARFS 3F1] (ab11038)This image is courtesy of an anonymous Abreviewab11038 detecting ARF1 in AGS Human gastric carcinoma cells by sandwich ELISA (detection). Samples were blocked with 5% BSA for 1 hour at 23°C and were incubated with the primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. Anti-mouse HRP (ab6728) (1/1000) was used as the secondary antibody.
The Capture antibody was ab436 - a mouse anti-human IgG (20 ug/ml, 4°C overnight) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038)](https://www.abcam.com/ps/products/11/ab11038/Images/ab11038-18717-anti-arf1-antibody-arfs-3f1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038)IHC image of ab11038 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11038, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Immunocytochemistry/ Immunofluorescence - Anti-ARF1 antibody [ARFS 3F1] (ab11038)](https://www.abcam.com/ps/products/11/ab11038/Images/ab11038-18716-anti-arf1-antibody-arfs-3f1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ARF1 antibody [ARFS 3F1] (ab11038)This image is courtesy of an Abreview submitted by Armen Petrosyanab11038 staining ARF1 in the Panc-1 cell line from Human pancreatic carcinoma by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Formalddehyde and blocked with 1% Donkey serum in PBST for 60 minutes at 22°C. Samples were incubated with primary antibody (1/50 in PBST + 1% Donkey serum) for 1 hour at 22°C. An Alexa Fluor®488-conjugated Donkey anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Datasheets and documents
References
ab11038 has been referenced in 7 publications.
- Xu J & Zhang J LncRNA TP73-AS1 is a novel regulator in cervical cancer via miR-329-3p/ARF1 axis. J Cell Biochem 121:344-352 (2020). PubMed: 31232491
- Pye DS et al. Chemokine unresponsiveness of chronic lymphocytic leukemia cells results from impaired endosomal recycling of Rap1 and is associated with a distinctive type of immunological anergy. J Immunol 191:1496-504 (2013). PubMed: 23804711
- Lim J et al. The CNK1 scaffold binds cytohesins and promotes insulin pathway signaling. Genes Dev 24:1496-506 (2010). WB ; Human . PubMed: 20634316
- Tang D et al. Molecular mechanism of mitotic Golgi disassembly and reassembly revealed by a defined reconstitution assay. J Biol Chem 283:6085-94 (2008). WB . PubMed: 18156178
- Havrylov S et al. Adaptor protein Ruk/CIN85 is associated with a subset of COPI-coated membranes of the Golgi complex. Traffic 9:798-812 (2008). PubMed: 18266907
- Cotton M et al. Endogenous ARF6 interacts with Rac1 upon angiotensin II stimulation to regulate membrane ruffling and cell migration. Mol Biol Cell 18:501-11 (2007). PubMed: 17122362
- Yano H et al. Fbx8 Makes Arf6 Refractory to Function via Ubiquitination. Mol Biol Cell : (2007). WB ; Human . PubMed: 18094045
