- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-ARF1 antibody [ARFS 3F1]
See all ARF1 primary antibodies -
DescriptionMouse monoclonal [ARFS 3F1] to ARF1
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Host speciesMouse
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SpecificityThis antibody is specific to class I ARF.
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Tested applicationsSuitable for: IHC-P, ICC/IF, ELISA, WBmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide:
SNQLRNQ
, corresponding to C terminal amino acids 174-180 of Human ARF1.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberARFS 3F1
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MyelomaSp2/0-Ag14
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IsotypeIgG2a
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Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab11038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/50. | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. |
Target
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FunctionGTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
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Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
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Cellular localizationGolgi apparatus. Cytoplasm > perinuclear region.
- Information by UniProt
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Database links
- Entrez Gene: 375 Human
- Omim: 103180 Human
- SwissProt: P84077 Human
- Unigene: 286221 Human
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Alternative names
- ADP Ribosylation Factor 1 antibody
- ADP-ribosylation factor 1 antibody
- ARF 1 antibody
see all
Images
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![ELISA - Anti-ARF1 antibody [ARFS 3F1] (ab11038)](https://www.abcam.com/ps/products/11/ab11038/Images/ARF1-Primary-antibodies-ab11038-4.jpg)
ELISA - Anti-ARF1 antibody [ARFS 3F1] (ab11038)This image is courtesy of an anonymous Abreviewab11038 detecting ARF1 in AGS Human gastric carcinoma cells by sandwich ELISA (detection). Samples were blocked with 5% BSA for 1 hour at 23°C and were incubated with the primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. Anti-mouse HRP (ab6728) (1/1000) was used as the secondary antibody.
The Capture antibody was ab436 - a mouse anti-human IgG (20 ug/ml, 4°C overnight) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038)](https://www.abcam.com/ps/products/11/ab11038/Images/ARF1-Primary-antibodies-ab11038-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038)IHC image of ab11038 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11038, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Immunocytochemistry/ Immunofluorescence - Anti-ARF1 antibody [ARFS 3F1] (ab11038)](https://www.abcam.com/ps/products/11/ab11038/Images/ARF1-Primary-antibodies-ab11038-14.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ARF1 antibody [ARFS 3F1] (ab11038)This image is courtesy of an Abreview submitted by Armen Petrosyanab11038 staining ARF1 in the Panc-1 cell line from Human pancreatic carcinoma by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Formalddehyde and blocked with 1% Donkey serum in PBST for 60 minutes at 22°C. Samples were incubated with primary antibody (1/50 in PBST + 1% Donkey serum) for 1 hour at 22°C. An Alexa Fluor®488-conjugated Donkey anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Pye DS et al. Chemokine unresponsiveness of chronic lymphocytic leukemia cells results from impaired endosomal recycling of Rap1 and is associated with a distinctive type of immunological anergy. J Immunol 191:1496-504 (2013). Read more (PubMed: 23804711) »
- Lim J et al. The CNK1 scaffold binds cytohesins and promotes insulin pathway signaling. Genes Dev 24:1496-506 (2010). WB ; Human . Read more (PubMed: 20634316) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"