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  1. Link

    arf1-antibody-arfs-3f1-ab11038.pdf

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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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Anti-ARF1 antibody [ARFS 3F1] (ab11038)

  • Datasheet
Reviews (6)Q&A (4)References (8)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038)

    Key features and details

    • Mouse monoclonal [ARFS 3F1] to ARF1
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG2a

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    Overview

    • Product name

      Anti-ARF1 antibody [ARFS 3F1]
      See all ARF1 primary antibodies
    • Description

      Mouse monoclonal [ARFS 3F1] to ARF1
    • Host species

      Mouse
    • Specificity

      This antibody is specific to class I ARF.
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide:

      SNQLRNQ

      , corresponding to C terminal amino acids 174-180 of Human ARF1.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • BALB/3T3 whole cell lysate (ab7901) can be used as a positive control in WB.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      ARFS 3F1
    • Myeloma

      Sp2/0-Ag14
    • Isotype

      IgG2a
    • Research areas

      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • Regulation
      • Signal Transduction
      • Protein Trafficking
      • ER Proteins

    Associated products

    • Assay kits

      • Arf1 Activation Assay Kit (ab211170)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Positive Controls

      • BALB/3T3 whole cell lysate (ab7901)
    • Recombinant Protein

      • Recombinant Human ARF1 protein (Tagged) (ab235860)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab11038 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
    • Sequence similarities

      Belongs to the small GTPase superfamily. Arf family.
    • Cellular localization

      Golgi apparatus. Cytoplasm > perinuclear region.
    • Target information above from: UniProt accession P84077 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 375 Human
      • Omim: 103180 Human
      • SwissProt: P84077 Human
      • Unigene: 286221 Human
      • Alternative names

        • ADP Ribosylation Factor 1 antibody
        • ADP-ribosylation factor 1 antibody
        • ARF 1 antibody
        • ARF1 antibody
        • ARF1_HUMAN antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038)
        IHC image of ab11038 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11038, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (8)

      Publishing research using ab11038? Please let us know so that we can cite the reference in this datasheet.

      ab11038 has been referenced in 8 publications.

      • Xu J & Zhang J LncRNA TP73-AS1 is a novel regulator in cervical cancer via miR-329-3p/ARF1 axis. J Cell Biochem 121:344-352 (2020). PubMed: 31232491
      • Tan JZA  et al. Distinct anterograde trafficking pathways of BACE1 and amyloid precursor protein from the TGN and the regulation of amyloid-ß production. Mol Biol Cell 31:27-44 (2020). PubMed: 31746668
      • Pye DS  et al. Chemokine unresponsiveness of chronic lymphocytic leukemia cells results from impaired endosomal recycling of Rap1 and is associated with a distinctive type of immunological anergy. J Immunol 191:1496-504 (2013). PubMed: 23804711
      • Lim J  et al. The CNK1 scaffold binds cytohesins and promotes insulin pathway signaling. Genes Dev 24:1496-506 (2010). WB ; Human . PubMed: 20634316
      • Tang D  et al. Molecular mechanism of mitotic Golgi disassembly and reassembly revealed by a defined reconstitution assay. J Biol Chem 283:6085-94 (2008). WB . PubMed: 18156178
      • Havrylov S  et al. Adaptor protein Ruk/CIN85 is associated with a subset of COPI-coated membranes of the Golgi complex. Traffic 9:798-812 (2008). PubMed: 18266907
      • Cotton M  et al. Endogenous ARF6 interacts with Rac1 upon angiotensin II stimulation to regulate membrane ruffling and cell migration. Mol Biol Cell 18:501-11 (2007). PubMed: 17122362
      • Yano H  et al. Fbx8 Makes Arf6 Refractory to Function via Ubiquitination. Mol Biol Cell : (2007). WB ; Human . PubMed: 18094045

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 10 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-ARF1 antibody [ARFS 3F1]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cell (Rat hepatocytes)
      Permeabilization
      Yes - 0.2% Triton X-100 in PBS
      Specification
      Rat hepatocytes
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Armen Petrosyan

      Verified customer

      Submitted Sep 08 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-ARF1 antibody [ARFS 3F1]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (Mice Hepatocytes)
      Permeabilization
      Yes - 0.2% Triton X-100 in PBS
      Specification
      Mice Hepatocytes
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 1°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Armen Petrosyan

      Verified customer

      Submitted Apr 09 2019

      Immunocytochemistry/ Immunofluorescence abreview for Anti-ARF1 antibody [ARFS 3F1]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Panc-1)
      Specification
      Panc-1
      Fixative
      Formaldehyde
      Permeabilization
      No
      Blocking step
      1% Donkey serum in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Armen Petrosyan

      Verified customer

      Submitted Dec 05 2012

      ELISA abreview for Anti-ARF1 antibody [ARFS 3F1]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      ELISA
      Sample
      Human Cell (AGS human gastric carcinoma cells)
      Specification
      AGS human gastric carcinoma cells
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Type
      Sandwich (Detection)
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Sep 06 2010

      Western blot abreview for Anti-ARF1 antibody [ARFS 3F1]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (AGS Gastric Carcinoma cells)
      Loading amount
      10 µg
      Specification
      AGS Gastric Carcinoma cells
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Jun 18 2010

      Western blot abreview for Anti-ARF1 antibody [ARFS 3F1]

      Below Average
      Abreviews
      Abreviews
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeLa)
      Loading amount
      50 µg
      Specification
      HeLa
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Apr 05 2007

      Question


      Inquiry: Multiple bands were detected (at ˜80, 50, and 35 kDa) and I only saw the expected perturbations in the band at 80 kD. Amount of protein loaded Human Cell lysate - whole cell (HL-60) unknown amount Human Cell lysate - pulldown with GST-GGA3-PBD (HL-60) unknown amount Blocking conditions Odyssey Casein blocking buffer 1 hr at room temp Detection method ECL Electrophoresis and transfer conditions Semi-dry transfer 30 min at 120 mA Primary antibody conditions (diluent/dilution, incubation time/temperature) 1:400 in 50/50 TBST/Casein blocking buffer overnight at 4degC Secondary antibody conditions (diluent/dilution, incubation time/temperature) ECL Anti-mouse IgG, Horseradish Peroxidase linked Conjugation: HRP 1:10000 in TBST,m 1 hr at room temp Wash steps 5 x 8 min TBST, one quick wash in TBS, one quick wash H2O

      Read More

      Abcam community

      Verified customer

      Asked on Sep 21 2012

      Answer

      Thank you for contacting us.

      I am sorry to hear that you are experiencing issues with this. I was wondering if you could help me to understand this problem by providing a few more details.

      Do you know the amount of total protein you are loading per well?

      Are these experiments being run under reduced and denatured or native conditions?

      Have you attempted to use any different concentrations of this antibody? The image on our site was created using 1:5000 use a 1:400 may be causing non specific bands.

      What perturbations are you expecting and how are these produced?

      Do you have any images?

      This product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue.



      I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Sep 21 2012

      Question

      Can you please check the queries ?

      Regards


      Dear Sir/Madam,

      I am a student. We had purchased ARFS-3F1 anti-ARF1 antibody (Abcam,cataloge no.-ab11038) from you. I wanted to equire about the exact size at which the antibody is expected to detect ARF1 in a western blot.

      Thank you

      Read More

      Abcam community

      Verified customer

      Asked on Mar 13 2012

      Answer

      Thank you for your enquiry,

      I can confirm the ARF1 protein has a molecular weight of around 20 kDa. There is a Western blotAbreview published on the online datasheet, including an image which shows aband at about 20 kDa.

      https://www.abcam.com/ARF1-antibody-ARFS-3F1-ab11038.html&tab=abreviews&intabreviewid=19226

      I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

      Read More

      Abcam Scientific Support

      Answered on Mar 13 2012

      Question

      Does your ARF1 antibody cross-react with other ARF isoforms?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 02 2005

      Answer

      Thank you for your enquiry. The originator of ab11038 has told me that this antibody is class 1 ARFS specific. If you have any additional questions, please contact us again.

      Read More

      Abcam Scientific Support

      Answered on Mar 10 2005

      Question

      Can you tell me any more about ab 11038 , ARF1 antibody? Like, does it recognise other ARFs? do you have any images of its behaviour in Westerns? has anything been published using this or "a very similar Ab"?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 11 2004

      Answer

      Thank you for your enquiry. All the information that we have regarding ab11038 is located on the online datasheet. Unfortunately, we are not aware of any publications that feature the use of this antibody and do not have any images to provide at this time.

      Read More

      Abcam Scientific Support

      Answered on Mar 12 2004

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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