Product nameAnti-ARF4 antibody [EPR12133(B)]
See all ARF4 primary antibodies
DescriptionRabbit monoclonal [EPR12133(B)] to ARF4
SpecificityMay cross react with family members, immunogen has 86% homology with ARF5
Tested applicationsSuitable for: WB, ICC/IF, IHC-P, IP, Flow Cytmore details
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human ARF4 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P18085
- HT-29, HepG2, K562 and HeLa whole cell lysate (ab150035); Human kidney and testis tissues; HepG2 cells; permeabilized K562 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 21 kDa.|
|ICC/IF||1/100 - 1/250.|
|IHC-P||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionGTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
Cellular localizationGolgi apparatus.
- Information by UniProt
- ADP ribosylation factor 2 antibody
- ADP ribosylation factor 4 antibody
- ADP-ribosylation factor 4 antibody
All lanes : Anti-ARF4 antibody [EPR12133(B)] (ab171746) at 1/10000 dilution
Lane 1 : HT-29 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 21 kDa
Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cell lysate or (Lane 2) 1X PBS (negative control) using ab171746 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunohistochemistry of paraffin-embedded Human testis tissue labeling ARF4 using ab171746 at 1/100 dilution.
Immunohistochemistry of paraffin-embedded Human kidney tissue labeling ARF4 using ab171746 at 1/100 dilution.
Immunofluorescence analysis of HepG2 cells labeling ARF4 using ab171746 at 1/100 dilution (green). DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized K562 cells labeling ARF4 using ab171746 (red) at 1/10 dilution, or a rabbit IgG (negative) (green).
ab171746 has not yet been referenced specifically in any publications.