Overview

  • Product name

  • Description

    Rabbit polyclonal to ARF5
  • Host species

    Rabbit
  • Specificity

    This antibody may cross react with close family members. The immunogen has 94% homology with ARF4, and 88% homology with ARF1 and 3.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 49-113 of Human ARF5

  • Positive control

    • Molt-4, Raji cells lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab96389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.

Target

  • Function

    GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Arf family.
  • Cellular localization

    Golgi apparatus. Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP ribosylation factor 5 antibody
    • ADP-ribosylation factor 5 antibody
    • ARF 5 antibody
    • Arf5 antibody
    • ARF5_HUMAN antibody
    see all

Images

  • All lanes : Anti-ARF5 antibody (ab96389) at 1/1500 dilution

    Lane 1 : Molt-4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 21 kDa



    15% SDS PAGE

References

ab96389 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (kidney, epithelial cells)
Loading amount
100000 cells
Specification
kidney, epithelial cells
Treatment
transfected (or not) with ARF5-HA and infected (or not) with influenza A virus
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 18°C

Dr. Maria Amorim

Verified customer

Submitted Apr 05 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney, epithelial cells)
Specification
kidney, epithelial cells
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C

Dr. Maria Amorim

Verified customer

Submitted Apr 03 2012

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