Product nameArf6 Activation Assay Kit
Sample typeTissue, Adherent cells, Suspension cells
Species reactivityReacts with: Rat, Cow, Dog, Human
Arf6 Activation Assay Kit (ab211171) uses GGA3 PBD Agarose beads to selectively isolate and pull-down the active form of Arf from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Arf is detected by western blot analysis using an anti-Arf6 antibody, which detects the human, rat, cow and dog protein.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
Arf6 (ADP-ribosylation factor 6) is a 20 kDa protein, member of ARF gene family, which constitutes part of the Ras superfamily. The Arf proteis are categorized as class I (Arf1, Arf2, Arf3), class II (Arf4 and Arf5) and class III (Arf6), and they all share a common gene organization. Arf6 regulates a variety of biological response pathways that include protein trafficking, organelle structure, and cytoskeleton remodeling. Like other small GTPases, Arf6 cycles between an inactive GDP-bound form and an active GTP-bound form: in its active (GTP-bound) state, Arf1 binds specifically to the protein-binding domain (PBD) of GGA3 to control downstream signaling cascades.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 20 tests 5 tests ab211180 - GGA3 PBD Agarose Beads 1 x 800µl 1 x 200µl 100X GTPyS 1 x 50µl 1 x 20µl 100X GDP 1 x 50µl 1 x 20µl 5X Assay/Lysis Buffer 1 x 30ml 4 x 2ml anti-Arf6 Mouse monoclonal antibody 1 x 40µl 1 x 10µl GTPase immunoblot Positive Control 1 x 100µl 1 x 100µl
FunctionGTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development (By similarity). Contributes to the regulation of dendritic branching and filopodia extension.
Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
Cellular localizationGolgi apparatus. Cell membrane. Endosome membrane. Cell membrane. Cell projection > filopodium membrane. Recruited to the cell membrane in association with CYTH2 and ARL4C.
- Information by UniProt
- ADP ribosylation factor 6
- ADP ribosylation factor protein 6
- ADP-ribosylation factor 6
ab211171 has not yet been referenced specifically in any publications.