Overview

  • Product name

    Anti-Arfaptin-1 antibody [EPR16360]
    See all Arfaptin-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16360] to Arfaptin-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human Arfaptin-1 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P53367

  • Positive control

    • HeLa, HepG2, BxPC-3, Jurkat, Human fetal liver, Mouse brain, Rat brain and Raw264.7 lysates; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 42 kDa).

Target

Images

  • All lanes : Anti-Arfaptin-1 antibody [EPR16360] (ab191430) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : HepG2 lysate
    Lane 3 : BxPC-3 lysate
    Lane 4 : Jurkat lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.

  • Anti-Arfaptin-1 antibody [EPR16360] (ab191430) at 1/10000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa
    Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.

  • All lanes : Anti-Arfaptin-1 antibody [EPR16360] (ab191430) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Raw264.7 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.

  • Flow Cytometrical analysis of Jurkat cells labeling Arfaptin-1 with ab191430 at 1/120 compared to a Isotype control. FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis.

References

ab191430 has not yet been referenced specifically in any publications.

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