Anti-ARFGAP1 antibody (ab85971)
Key features and details
- Rabbit polyclonal to ARFGAP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARFGAP1 antibody -
Description
Rabbit polyclonal to ARFGAP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human ARFGAP1 aa 466-516.
Database link: NP_055385.2 -
Positive control
- HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab85971 was affinity purified using an epitope specific to ARFGAP1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. -
Tissue specificity
Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary. -
Sequence similarities
Contains 1 Arf-GAP domain. -
Developmental stage
Expressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. Expressed at lower level in spleen, heart, kidney and liver during development. -
Cellular localization
Cytoplasm. Golgi apparatus membrane. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment. - Information by UniProt
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Database links
- Entrez Gene: 458885 Chimpanzee
- Entrez Gene: 26286 Human
- Entrez Gene: 711160 Rhesus monkey
- Omim: 612439 Human
- SwissProt: Q9NP61 Human
- SwissProt: Q5R787 Orangutan
- Unigene: 685225 Human
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Alternative names
- ADP ribosylation factor GTPase activating protein 1 antibody
- ADP ribosylation factor GTPase activating protein 3 antibody
- ADP-ribosylation factor GTPase-activating protein 3 antibody
see all
Images
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All lanes : Anti-ARFGAP1 antibody (ab85971) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 57 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Detection of ARFGAP1 by Western Blot of Immunprecipitate.
ab85971 at 1µg/ml staining ARFGAP1 in HeLa whole cell lysate immunoprecipitated using ab85971 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab85971 has been referenced in 1 publication.
- Anthony DF et al. {beta}-Arrestin 1 Inhibits the GTPase-Activating Protein Function of ARHGAP21, Promoting Activation of RhoA following Angiotensin II Type 1A Receptor Stimulation. Mol Cell Biol 31:1066-75 (2011). WB ; Human . PubMed: 21173159