Overview

  • Product name

  • Description

    Rabbit polyclonal to ARFGAP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ARFGAP1 aa 466-516.
    Database link: NP_055385.2

  • Positive control

    • HeLa whole cell lysate.
  • General notes

     This product was previously labelled as ARFGAP3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab85971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
  • Tissue specificity

    Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.
  • Sequence similarities

    Contains 1 Arf-GAP domain.
  • Developmental stage

    Expressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. Expressed at lower level in spleen, heart, kidney and liver during development.
  • Cellular localization

    Cytoplasm. Golgi apparatus membrane. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP ribosylation factor GTPase activating protein 1 antibody
    • ADP ribosylation factor GTPase activating protein 3 antibody
    • ADP-ribosylation factor GTPase-activating protein 3 antibody
    • ARF GAP 3 antibody
    • ARFG3_HUMAN antibody
    • ArfGAP 3 antibody
    • ARFGAP1 antibody
    • ARFGAP3 antibody
    • FLJ45618 antibody
    see all

Images

  • All lanes : Anti-ARFGAP1 antibody (ab85971) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Predicted band size: 57 kDa
    Observed band size: 63 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • Detection of ARFGAP1 by Western Blot of Immunprecipitate.
    ab85971 at 1µg/ml staining ARFGAP1 in HeLa whole cell lysate immunoprecipitated using ab85971 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

References

This product has been referenced in:

  • Anthony DF  et al. {beta}-Arrestin 1 Inhibits the GTPase-Activating Protein Function of ARHGAP21, Promoting Activation of RhoA following Angiotensin II Type 1A Receptor Stimulation. Mol Cell Biol 31:1066-75 (2011). WB ; Human . Read more (PubMed: 21173159) »
See 1 Publication for this product

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