Overview

  • Product name

    Anti-ARFGAP1 antibody [EPR13650]
    See all ARFGAP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13650] to ARFGAP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ARFGAP1 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8N6T3

  • Positive control

    • Human fetal brain lysate; HepG2, HeLa and MCF7 cell lysates; HeLa and MCF7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF 1/500.

Target

  • Function

    GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and RNP24. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine.
  • Sequence similarities

    Contains 1 Arf-GAP domain.
  • Domain

    The region downstream of Arf-GAP domain is essential to GAP activity in vivo. This region may be required for its targeting to Golgi membranes.
  • Cellular localization

    Cytoplasm. Golgi apparatus. Associates with the Golgi complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP ribosylation factor 1 GTPase activating protein antibody
    • ADP ribosylation factor GTPase activating protein 1 antibody
    • ADP-ribosylation factor 1 GTPase-activating protein antibody
    • ADP-ribosylation factor GTPase-activating protein 1 antibody
    • ARF GAP 1 antibody
    • ARF1 directed GTPase activating protein antibody
    • ARF1 GAP antibody
    • ARF1-directed GTPase-activating protein antibody
    • ARF1GAP antibody
    • ARFG1_HUMAN antibody
    • Arfgap1 antibody
    • GAP protein antibody
    • HRIHFB2281 antibody
    see all

Images

  • All lanes : Anti-ARFGAP1 antibody [EPR13650] (ab183746) at 1/5000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • All lanes : Anti-ARFGAP1 antibody [EPR13650] (ab183746) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Immunofluorescent analysis of acetone-fixed HeLa cells labeling ARFGAP1 with ab183746 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling ARFGAP1 with ab183746 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).

References

This product has been referenced in:

  • Chornyy S  et al. High abundance of ArfGAP1 found in the mossy fibers in hilus of the dentate gyrus region of the mouse brain. PLoS One 12:e0189659 (2017). Read more (PubMed: 29240824) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183746.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up