Overview

  • Product name

    Anti-ARFGEF2/BIG2 antibody
    See all ARFGEF2/BIG2 primary antibodies
  • Description

    Rabbit polyclonal to ARFGEF2/BIG2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ARFGEF2/BIG2.
    Database link: NP_006411.1

  • Positive control

    • HeLa whole cell lysate.
  • General notes

     This product was previously labelled as ARFGEF2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab72420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 202 kDa (predicted molecular weight: 202 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6 and the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.
  • Cellular localization

    Cytoplasm. Membrane. Golgi apparatus. Cytoplasm › perinuclear region. Golgi apparatus › trans-Golgi network By similarity. Endosome By similarity. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cell projection › dendrite By similarity. Cytoplasmic vesicle By similarity. Cell junction › synapse By similarity. Cytoplasm › cytoskeleton By similarity. Note: Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.
  • Database links

  • Alternative names

    • ADP ribosylation factor guanine nucleotide exchange factor 2 (brefeldin A inhibited) antibody
    • ADP ribosylation factor guanine nucleotide exchange factor 2 antibody
    • ARFGEF 2 antibody
    • ARFGEF2 antibody
    • ARFGEP2 antibody
    • BIG 2 antibody
    • BIG2 antibody
    • Brefeldin A inhibited 2 antibody
    • Brefeldin A inhibited GEP 2 antibody
    • Brefeldin A inhibited guanine nucleotide exchange protein 2 antibody
    • dJ1164I10.1 antibody
    • FLJ23723 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ARFGEF2/BIG with ab72420 at 1/200 (1µg/ml). Detection: DAB.

  • All lanes : Anti-ARFGEF2/BIG2 antibody (ab72420) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 202 kDa
    Observed band size: 202 kDa


    Exposure time: 3 minutes
  • Detection of ARFGEF2/BIG by Western Blot of Immunprecipitate.
    ab72420 at 1µg/ml staining ARFGEF2/BIG in HeLa whole cell lysate immunoprecipitated using ab72420 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab72420 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Rat Cell lysate - whole cell (Vascular Smooth Muscle Cells)
Gel Running Conditions
Reduced Denaturing (4-20% Gradient)
Loading amount
10 µg
Specification
Vascular Smooth Muscle Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 24 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Vascular Smooth Muscle Cells)
Antigen retrieval step
None
Permeabilization
Yes - PBS+0.25% Triton X-100 (10 mins)
Specification
Vascular Smooth Muscle Cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Paraformaldehyde

Michael Morran

Verified customer

Submitted May 18 2018

Question
Answer

We testedab72420 for reactivity to mouse protein and found it did not react.

I hope this is helpful. Please contact me again if you have any further questions.

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