Product nameAnti-ARFGEF2/BIG2 antibody
See all ARFGEF2/BIG2 primary antibodies
DescriptionRabbit polyclonal to ARFGEF2/BIG2
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human ARFGEF2/BIG2.
Database link: NP_006411.1
- HeLa whole cell lysate.
This product was previously labelled as ARFGEF2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72420 was affinity purified using an epitope specific to ARFGEF2/BIG2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab72420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 202 kDa (predicted molecular weight: 202 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6 and the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.
Cellular localizationCytoplasm. Membrane. Golgi apparatus. Cytoplasm › perinuclear region. Golgi apparatus › trans-Golgi network By similarity. Endosome By similarity. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cell projection › dendrite By similarity. Cytoplasmic vesicle By similarity. Cell junction › synapse By similarity. Cytoplasm › cytoskeleton By similarity. Note: Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.
- ADP ribosylation factor guanine nucleotide exchange factor 2 (brefeldin A inhibited) antibody
- ADP ribosylation factor guanine nucleotide exchange factor 2 antibody
- ARFGEF 2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ARFGEF2/BIG with ab72420 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-ARFGEF2/BIG2 antibody (ab72420) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 202 kDa
Observed band size: 202 kDa
Exposure time: 3 minutes
Detection of ARFGEF2/BIG by Western Blot of Immunprecipitate.
ab72420 at 1µg/ml staining ARFGEF2/BIG in HeLa whole cell lysate immunoprecipitated using ab72420 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab72420 has not yet been referenced specifically in any publications.