Overview

  • Product name
    Anti-ARFRP1 antibody [EPR3899]
    See all ARFRP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3899] to ARFRP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICCmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ARFRP1 aa 150-250. The exact sequence is proprietary.

  • Positive control
    • WB: HepG2 and HEK-293T cell lysate. Fetal liver and ovary cancer lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR3899
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).
IP 1/10 - 1/100.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    Images

    • All lanes : Anti-ARFRP1 antibody [EPR3899] (ab108199) at 1/1000 dilution

      Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
      Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
      Lane 3 : Fetal liver lysate
      Lane 4 : Ovary cancer lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 23 kDa

    References

    This product has been referenced in:
    • Rödiger M  et al. Adiponectin release and insulin receptor targeting share trans-Golgi-dependent endosomal trafficking routes. Mol Metab 8:167-179 (2018). WB, ICC/IF ; Human . Read more (PubMed: 29203237) »
    • Covington JD  et al. Skeletal muscle perilipin 3 and coatomer proteins are increased following exercise and are associated with fat oxidation. PLoS One 9:e91675 (2014). WB ; Human . Read more (PubMed: 24632837) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab108199.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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