Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3899] to ARFRP1/ARP
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-ARFRP1/ARP antibody [EPR3899]
See all ARFRP1/ARP primary antibodies
DescriptionRabbit monoclonal [EPR3899] to ARFRP1/ARP
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ARFRP1/ARP aa 150-250. The exact sequence is proprietary.
- WB: HepG2 and HEK-293T cell lysate. Fetal liver and ovary cancer lysates ICC/IF: HeLa cells
Previously labelled as ARFRP1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab108199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).|
FunctionPossibly involved in plasma membrane-related signaling events.
Tissue specificityFound in most tissues.
Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
Cellular localizationGolgi apparatus.
- Information by UniProt
- ADP ribosylation factor related protein 1 antibody
- ADP-ribosylation factor-related protein 1 antibody
- ARF related protein 1 antibody
All lanes : Anti-ARFRP1/ARP antibody [EPR3899] (ab108199) at 1/1000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Fetal liver lysate
Lane 4 : Ovary cancer lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa
Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ARFRP1/ARP with purified ab108199 at 1/500 dilution (4.8 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
ab108199 has been referenced in 2 publications.
- Rödiger M et al. Adiponectin release and insulin receptor targeting share trans-Golgi-dependent endosomal trafficking routes. Mol Metab 8:167-179 (2018). WB, ICC/IF ; Human . PubMed: 29203237
- Covington JD et al. Skeletal muscle perilipin 3 and coatomer proteins are increased following exercise and are associated with fat oxidation. PLoS One 9:e91675 (2014). WB ; Human . PubMed: 24632837