Key features and details
- Rabbit polyclonal to Arg2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Arg2 antibody
See all Arg2 primary antibodies
DescriptionRabbit polyclonal to Arg2
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Recombinant full length protein corresponding to Human Arg2 aa 1-354.
MSLRGSLSRLLQTRVHSILKKSVHSVAVIGAPFSQGQKRKGVEHGPAAIR EAGLMKRLSSLGCHLKDFGDLSFTPVPKDDLYNNLIVNPRSVGLANQELA EVVSRAVSDGYSCVTLGGDHSLAIGTISGHARHCPDLCVVWVDAHADINT PLTTSSGNLHGQPVSFLLRELQDKVPQLPGFSWIKPCISSASIVYIGLRD VDPPEHFILKNYDIQYFSMRDIDRLGIQKVMERTFDLLIGKRQRPIHLSF DIDAFDPTLAPATGTPVVGGLTYREGMYIAEEIHNTGLLSALDLVEVNPQ LATSEEEAKTTANLAVDVIASSFGQTREGGHIVYDQLPTPSSPDESENQA RVRI
Database link: P78540
- WB: Mouse liver and kidney tissue lysates; HEK-293T whole cell lysate. IHC-P: Human lung cancer and prostate tissues. IP: HEK-293 whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab235944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 39 kDa.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
FunctionMay play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders.
Tissue specificityExpressed most strongly in kidney and prostate, much less strongly in the brain, skeletal muscle, placenta, lung, mammary gland, macrophage, uterus, testis and gut, but apparently not in the liver, heart and pancreas.
PathwayNitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Sequence similaritiesBelongs to the arginase family.
- Information by UniProt
- ARG2 antibody
- ARGI2_HUMAN antibody
- Arginase II mitochondrial antibody
All lanes : Anti-Arg2 antibody (ab235944) at 1/500 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Mouse kidney tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 39 kDa
Paraffin-embedded human prostate tissue stained for Arg2 using ab235944 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human lung cancer tissue stained for Arg2 using ab235944 at 1/100 dilution in immunohistochemical analysis.
Arg2 was immunoprecipitated from HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate using ab235944 at 1/200 dilution. For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
Lane 1: Rabbit control IgG (1 μg) instead of ab235944 in HEK-293 whole cell lysate.
Lane 2: ab235944 IP in HEK-293 whole cell lysate (500 μg).
Lane 3: HEK-293 whole cell lysate (input; 10 μg)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235944 has not yet been referenced specifically in any publications.