Recombinant
RabMAb

Recombinant Anti-Arg2 antibody [EPR22626-51] (ab228963)

Overview

  • Product name

    Anti-Arg2 antibody [EPR22626-51]
    See all Arg2 primary antibodies
  • Description

    Rabbit monoclonal [EPR22626-51] to Arg2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: IHC-Fr
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse Arg2 aa 50-350. The exact sequence is proprietary.
    Database link: O08691

  • Positive control

    • WB: Mouse and rat small intestine and kidney lysate. IHC-P: Mouse small intestine tissue; rat kidney tissue. IP: Rat kidney lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab228963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.
  • Application notes
    Is unsuitable for IHC-Fr.
  • Target

    • Function

      May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders.
    • Tissue specificity

      Expressed most strongly in kidney and prostate, much less strongly in the brain, skeletal muscle, placenta, lung, mammary gland, macrophage, uterus, testis and gut, but apparently not in the liver, heart and pancreas.
    • Pathway

      Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
    • Sequence similarities

      Belongs to the arginase family.
    • Cellular localization

      Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • ARG2 antibody
      • ARGI2_HUMAN antibody
      • Arginase II mitochondrial antibody
      • Arginase type II antibody
      • Arginase-2 antibody
      • arginase-2, mitochondrial antibody
      • Kidney arginase antibody
      • Kidney type arginase antibody
      • Kidney-type arginase antibody
      • L arginine amidinohydrolase antibody
      • L arginine ureahydrolase antibody
      • mitochondrial antibody
      • Non hepatic arginase antibody
      • Non-hepatic arginase antibody
      • Nonhepatic arginase antibody
      • Type II arginase antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling Arg2 with ab228963 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the mouse small intestine (PMID: 19000307) is observed. The section was incubated with ab228963 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Arg2 was immunoprecipitated from 0.35 mg rat kidney lysate with ab228963 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab228963 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.

      Lane 1: Rat kidney lysate 10 µg (Input).
      Lane 2: ab228963 IP in rat kidney lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab228963 in rat kidney lysate.

      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 5 seconds.

    • Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Arg2 with ab228963 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the tubules in the rat kidney (PMID: 8954792) is observed. The section was incubated with ab228963 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • All lanes : Anti-Arg2 antibody [EPR22626-51] (ab228963) at 1/1000 dilution

      Lane 1 : Mouse small intestine lysate
      Lane 2 : Mouse kidney lysate
      Lane 3 : Mouse liver lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 39 kDa
      Observed band size: 39 kDa



      Negative control: Mouse liver (PMID: 21975054).

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lane 1: 10 seconds; Lanes 2-3: 3 minutes.

    • All lanes : Anti-Arg2 antibody [EPR22626-51] (ab228963) at 1/1000 dilution

      Lane 1 : Rat small intestine lysate
      Lane 2 : Rat kidney lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 39 kDa
      Observed band size: 39 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    References

    ab228963 has not yet been referenced specifically in any publications.

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