Overview

  • Product name

    Anti-Arg2 antibody [EPR9473]
    See all Arg2 primary antibodies
  • Description

    Rabbit monoclonal [EPR9473] to Arg2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Arg2 aa 300-400 (C terminal). The exact sequence is proprietary.
    Database link: P78540

  • Positive control

    • Caco 2, Jurkat and 293T cell lysates; Caco 2 cells; JAR whole cell lysates; HEK-293.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1: 100 - 1/10000.

 

WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.

Target

  • Function

    May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders.
  • Tissue specificity

    Expressed most strongly in kidney and prostate, much less strongly in the brain, skeletal muscle, placenta, lung, mammary gland, macrophage, uterus, testis and gut, but apparently not in the liver, heart and pancreas.
  • Pathway

    Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
  • Sequence similarities

    Belongs to the arginase family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARG2 antibody
    • ARGI2_HUMAN antibody
    • Arginase II mitochondrial antibody
    • Arginase type II antibody
    • Arginase-2 antibody
    • arginase-2, mitochondrial antibody
    • Kidney arginase antibody
    • Kidney type arginase antibody
    • Kidney-type arginase antibody
    • L arginine amidinohydrolase antibody
    • L arginine ureahydrolase antibody
    • mitochondrial antibody
    • Non hepatic arginase antibody
    • Non-hepatic arginase antibody
    • Nonhepatic arginase antibody
    • Type II arginase antibody
    see all

Images

  • Anti-Arg2 antibody [EPR9473] (ab137069) at 1/20000 dilution (purified) + Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa



    Blocking and diluting buffer : 5% NFDM/TBST
  • Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling Arg2 with purified ab137069 at 1:60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Arg2 with Purified ab137069 at 1:500 dilution (1.2 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Anti-Arg2 antibody [EPR9473] (ab137069) at 1/10000 dilution (purified) + JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa



    Blocking and diluting buffer : 5% NFDM/TBST
  • Immunofluorescent analysis of Caco 2 cells labelling Arg2 with ab137069 at 1/250 dilution.

  • Overlay histogram showing HEK293 cells stained with ab137069 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137069, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-Arg2 antibody [EPR9473] (ab137069) at 1/1000 dilution

    Lane 1 : Caco 2 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Common marmoset Cell lysate - whole cell (Kidney)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
45 µg
Treatment
Lane 2-4 control, lane 5-7 1 uM MeHg for 24 hrs
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Mr. Patrick Langlois

Verified customer

Submitted Jun 26 2017

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