Recombinant Anti-Arg2 antibody [EPR9473] (ab137069)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9473] to Arg2
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Arg2 antibody [EPR9473]
See all Arg2 primary antibodies -
Description
Rabbit monoclonal [EPR9473] to Arg2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Arg2 aa 300-400 (C terminal). The exact sequence is proprietary.
Database link: P78540 -
Positive control
- Caco 2, Jurkat and 293T cell lysates; Caco 2 cells; JAR whole cell lysates; HEK-293, GST tagged Recombinant Human ARG2 protein.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9473 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/60.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1: 100 - 1/10000. |
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ICC/IF |
1/250 - 1/500.
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
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Notes |
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Flow Cyt (Intra)
1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1: 100 - 1/10000. |
ICC/IF
1/250 - 1/500. |
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa. |
Target
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Function
May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders. -
Tissue specificity
Expressed most strongly in kidney and prostate, much less strongly in the brain, skeletal muscle, placenta, lung, mammary gland, macrophage, uterus, testis and gut, but apparently not in the liver, heart and pancreas. -
Pathway
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
Sequence similarities
Belongs to the arginase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 384 Human
- Omim: 107830 Human
- SwissProt: P78540 Human
- Unigene: 723133 Human
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Alternative names
- ARG2 antibody
- ARGI2_HUMAN antibody
- Arginase II mitochondrial antibody
see all
Images
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All lanes : Anti-Arg2 antibody [EPR9473] (ab137069) at 1/1000 dilution
Lane 1 : GST tagged Recombinant Human ARG1 protein (full-length, aa 1 to 322) (61 KDa)
Lane 2 : GST tagged Recombinant Human ARG2 protein (full-length, aa 1 to 354) (65 KDa)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
Anti-Arg2 antibody [EPR9473] (ab137069) at 1/20000 dilution (purified) + Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Blocking and diluting buffer : 5% NFDM/TBST -
Intracellular Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling Arg2 with purified ab137069 at 1/60 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Arg2 with Purified ab137069 at 1:500 dilution (1.2 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Anti-Arg2 antibody [EPR9473] (ab137069) at 1/10000 dilution (purified) + JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Blocking and diluting buffer : 5% NFDM/TBST -
Immunofluorescent analysis of Caco 2 cells labelling Arg2 with ab137069 at 1/250 dilution.
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Overlay histogram showing HEK293 cells stained with ab137069 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137069, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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All lanes : Anti-Arg2 antibody [EPR9473] (ab137069) at 1/1000 dilution
Lane 1 : Caco 2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab137069 has been referenced in 8 publications.
- Ito Y et al. NKX2-1 re-expression induces cell death through apoptosis and necrosis in dedifferentiated thyroid carcinoma cells. PLoS One 16:e0259558 (2021). PubMed: 34748583
- Grzywa TM et al. Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells. Commun Biol 4:1384 (2021). PubMed: 34893694
- Geck RC et al. Inhibition of the polyamine synthesis enzyme ornithine decarboxylase sensitizes triple-negative breast cancer cells to cytotoxic chemotherapy. J Biol Chem 295:6263-6277 (2020). PubMed: 32139506
- Lowe MM et al. Regulatory T cells use arginase 2 to enhance their metabolic fitness in tissues. JCI Insight 4:N/A (2019). PubMed: 31852848
- Roci I et al. Mapping Metabolic Events in the Cancer Cell Cycle Reveals Arginine Catabolism in the Committed SG2M Phase. Cell Rep 26:1691-1700.e5 (2019). PubMed: 30759381
- Tan JL et al. New High-Throughput Screening Identifies Compounds That Reduce Viability Specifically in Liver Cancer Cells That Express High Levels of SALL4 by Inhibiting Oxidative Phosphorylation. Gastroenterology 157:1615-1629.e17 (2019). PubMed: 31446059
- Zaytouni T et al. Critical role for arginase 2 in obesity-associated pancreatic cancer. Nat Commun 8:242 (2017). PubMed: 28808255
- Vanden Heuvel JP et al. Registered report: Systematic identification of genomic markers of drug sensitivity in cancer cells. Elife 5:N/A (2016). PubMed: 27336789