Overview

  • Product name

    Arg8-Vasopressin ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    32.1 pg/mL 22 14.3%
    70.7 pg/mL 22 6.7%
    143.7 pg/mL 22 6%
    Inter-assay
    Sample n Mean SD CV%
    33.0 13 9.5%
    66.4 13 6.4%
    136.2 13 8.6%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Competitive
  • Sensitivity

    2.84 pg/ml
  • Range

    4.1 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Culture Media 116.2 % - %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity


    Predicted to work with: a wide range of other species
  • Product overview

    Abcam’s Arg8-Vasopressin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Arg8-Vasopressin in tissue culture media.


    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with a Biotin conjugated- Arg8-Vasopressin antigen and a polyclonal rabbit antibody specific to Arg8-Vasopressin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Arg8-Vasopressin captured in the plate.

  • Notes

    Arginine Vasopressin (AVP) is a 9 amino acid peptide with a 6-member disulfide ring. It is structurally related to oxytocin differing in 2 amino acids. It is synthesized in the hypothalamus supraoptic and paraventricular nuclei. It is stored in the posterior pituitary for release. AVP has powerful antidiuretic action and is also known as antidiuretic hormone (ADH). It acts upon the collecting tubule of the kidney increasing permeability to water and urea. It also has neurotransmitter and peripheral humoral functions.

    AVP has been shown to be released upon both osmotic and non-osmotic stimuli, and its release into peripheral blood causes effects upon a number of factors, including emotional stress, posture, blood volume, and temperature. Alcohol appears to inhibit AVP secretion. Serum AVP measurement is used clinically for studies involving diabetes insipidus, syndrome of inappropriate ADH secretion (SIADH), ectopic AVP production and pyschogenic water intoxication.

     

     

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab205928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Arg8 - Vasopressin Typical Standard Curve

Protocols

References

This product has been referenced in:

  • Dayanithi G  et al. Vasopressin and oxytocin in sensory neurones: expression, exocytotic release and regulation by lactation. Sci Rep 8:13084 (2018). Read more (PubMed: 30166555) »
See 1 Publication for this product

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