Overview

  • Product name
    Arginase Activity Assay Kit (Colorimetric)
  • Detection method
    Colorimetric
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Sensitivity
    < 0.2 U/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mammals
  • Product overview

    Arginase Activity Assay (Colorimetric) (ab180877) kit is a simple, sensitive and rapid assay to quantify arginase activity. Arginase reacts with arginine and undergoes a series of reactions that form an intermediate that react stoichiometrically with the probe to generate a colored product that can detected at OD = 570 nm.

    The kit can detect less than 0.2 U/L Arginase activity in 96-well assay format.

  • Notes

    Arginase (EC 3.5.3.1) is the final enzyme of the Urea Cycle. It converts L-arginine into urea and L-ornithine and plays an important role in removing ammonium ion from the body. Arginase has two isoforms: Arginase I and Arginase II. Arginase I is mainly present in the liver and plays an important part in the urea cycle whereas Arginase II is present in kidney and other tissues and regulates Arginine/Ornithine concentration. Arginase deficiency can lead to severe symptoms including neurological impairment, dementia and hyperammonemia. Analysis of Arginase activity is fundamental to the study of the urea metabolic pathway.

  • Platform
    Reagents

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Arginase Assay Buffer WM 1 x 25ml
    Arginase Converter Enzyme (Lyophilized) Blue 1 vial
    Arginase Developer (Lyophilized) Orange 1 vial
    Arginase Enzyme Mix (Lyophilized) Green 1 vial
    Arginase Positive Control (Lyophilized) Purple 1 vial
    Arginase Substrate (Lyophilized) White 1 vial
    Hydrogen peroxide Yellow 1 x 100µl
    OxiRed Probe in DMSO Red 1 x 200µl
  • Pathway
    Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
  • Involvement in disease
    Argininemia
  • Sequence similarities
    Belongs to the arginase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Alternative names
    • arg1
    • ARGI1_HUMAN
    • Arginase-1
    • Liver-type arginase
    • Type I arginase
    see all

Associated products

Images

  • Arginase activity in rat liver lysate (3 µg) & Positive Control (2 µL). Assays were performed following the kit protocol.

Protocols

References

This product has been referenced in:
  • Das A  et al. Novel mechanisms of Collagenase Santyl Ointment (CSO) in wound macrophage polarization and resolution of wound inflammation. Sci Rep 8:1696 (2018). Read more (PubMed: 29374192) »
  • Yeku OO  et al. Armored CAR T cells enhance antitumor efficacy and overcome the tumor microenvironment. Sci Rep 7:10541 (2017). Read more (PubMed: 28874817) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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