Recombinant Anti-Arginine (glcnac ) antibody [EPR18251] (ab195033)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18251] to Arginine (glcnac )
- Suitable for: WB
- Reacts with: Species independent
Related conjugates and formulations
Overview
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Product name
Anti-Arginine (glcnac ) antibody [EPR18251]
See all Arginine primary antibodies -
Description
Rabbit monoclonal [EPR18251] to Arginine (glcnac ) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T cells transfected with TRADD and NleB whole cell lysate;
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General notes
This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Feng Shao, National Institute of Biological Sciences (NIBS), Beijing.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18251 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000.
NleB contained an unprecedented N-acetylglucosamine (GlcNAc) transferase activity that specifically modified a conserved arginine in these death domains (Arg 235 in the TRADD death domain). |
Notes |
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WB
1/2000. NleB contained an unprecedented N-acetylglucosamine (GlcNAc) transferase activity that specifically modified a conserved arginine in these death domains (Arg 235 in the TRADD death domain). |
Target
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Relevance
Arginine is an alpha amino acid. The L form is one of the 20 most common natural amino acids. In mammals, arginine is classified as a semi essential or conditionally essential amino acid, depending on the developmental stage and health status of the individual. -
Alternative names
- Arg antibody
- L arginine antibody
Images
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All lanes : Anti-Arginine (glcnac ) antibody [EPR18251] (ab195033) at 1/2000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TRADD
Lane 2 : HEK-293 whole cell lysate transfected with TRADD and NleB
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution5% NFDM/TBST: Blocking and dilution buffer.
The image was kindly provided by our collaborator Dr Feng Shao's lab, NIBS.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (13)
ab195033 has been referenced in 13 publications.
- Scott NE Glycopeptide-Centric Approaches for the Characterization of Microbial Glycoproteomes. Methods Mol Biol 2456:153-171 (2022). PubMed: 35612741
- Xue J et al. Arginine GlcNAcylation and Activity Regulation of PhoP by a Type III Secretion System Effector in Salmonella. Front Microbiol 12:825743 (2021). PubMed: 35126337
- El Qaidi S et al. An intra-bacterial activity for a T3SS effector. Sci Rep 10:1073 (2020). PubMed: 31974499
- Xue J et al. Auto Arginine-GlcNAcylation Is Crucial for Bacterial Pathogens in Regulating Host Cell Death. Front Cell Infect Microbiol 10:197 (2020). PubMed: 32432056
- Meng K et al. Arginine GlcNAcylation of Rab small GTPases by the pathogen Salmonella Typhimurium. Commun Biol 3:287 (2020). PubMed: 32504010