Product nameAnti-Arginine (glcnac ) antibody [EPR18251]
See all Arginine primary antibodies
DescriptionRabbit monoclonal [EPR18251] to Arginine (glcnac )
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Species independent
Synthetic peptide corresponding to Arginine (glcnac ).
- WB: 293T cells transfected with TRADD and NleB whole cell lysate;
This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Feng Shao, National Institute of Biological Sciences (NIBS), Beijing.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab195033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
NleB contained an unprecedented N-acetylglucosamine (GlcNAc) transferase activity that specifically modified a conserved arginine in these death domains (Arg 235 in the TRADD death domain).
RelevanceArginine is an alpha amino acid. The L form is one of the 20 most common natural amino acids. In mammals, arginine is classified as a semi essential or conditionally essential amino acid, depending on the developmental stage and health status of the individual.
- Arg antibody
- L arginine antibody
All lanes : Anti-Arginine (glcnac ) antibody [EPR18251] (ab195033) at 1/2000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TRADD
Lane 2 : HEK-293 whole cell lysate transfected with TRADD and NleB
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
5% NFDM/TBST: Blocking and dilution buffer.
WB image from Dr. Feng Shao's lab.
This product has been referenced in:
- Esposito D et al. Structural basis for the glycosyltransferase activity of the Salmonella effector SseK3. J Biol Chem 293:5064-5078 (2018). IP ; Human . Read more (PubMed: 29449376) »
- Xu C et al. A pathogen-derived effector modulates host glucose metabolism by arginine GlcNAcylation of HIF-1a protein. PLoS Pathog 14:e1007259 (2018). Read more (PubMed: 30125331) »