Recombinant
RabMAb

Recombinant Anti-Arginine (glcnac ) antibody [EPR18251] (ab195033)

Overview

  • Product name

    Anti-Arginine (glcnac ) antibody [EPR18251]
    See all Arginine primary antibodies
  • Description

    Rabbit monoclonal [EPR18251] to Arginine (glcnac )
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to Arginine (glcnac ).

  • Positive control

    • WB: 293T cells transfected with TRADD and NleB whole cell lysate;
  • General notes

    This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Feng Shao, National Institute of Biological Sciences (NIBS), Beijing.

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab195033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000.

NleB contained an unprecedented N-acetylglucosamine (GlcNAc) transferase activity that specifically modified a conserved arginine in these death domains (Arg 235 in the TRADD death domain).

Target

  • Relevance

    Arginine is an alpha amino acid. The L form is one of the 20 most common natural amino acids. In mammals, arginine is classified as a semi essential or conditionally essential amino acid, depending on the developmental stage and health status of the individual.
  • Alternative names

    • Arg antibody
    • L arginine antibody

Images

  • All lanes : Anti-Arginine (glcnac ) antibody [EPR18251] (ab195033) at 1/2000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TRADD
    Lane 2 : HEK-293 whole cell lysate transfected with TRADD and NleB

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution


    5% NFDM/TBST: Blocking and dilution buffer.

    WB image from Dr. Feng Shao's lab.

References

This product has been referenced in:

  • Esposito D  et al. Structural basis for the glycosyltransferase activity of the Salmonella effector SseK3. J Biol Chem 293:5064-5078 (2018). IP ; Human . Read more (PubMed: 29449376) »
  • Xu C  et al. A pathogen-derived effector modulates host glucose metabolism by arginine GlcNAcylation of HIF-1a protein. PLoS Pathog 14:e1007259 (2018). Read more (PubMed: 30125331) »
See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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