Overview

  • Product name

    Anti-Argininosuccinate Lyase antibody
    See all Argininosuccinate Lyase primary antibodies
  • Description

    Rabbit polyclonal to Argininosuccinate Lyase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 86-335 of Human Argininosuccinate Lyase.

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cells

Properties

Applications

Our Abpromise guarantee covers the use of ab96215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.

Target

  • Pathway

    Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.
    Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-L-arginino)succinate: step 1/1.
  • Involvement in disease

    Defects in ASL are the cause of arginosuccinicaciduria (ARGINSA) [MIM:207900]. Arginosuccinicaciduria is an autosomal recessive disorder of the urea cycle. The disease is characterized by mental and physical retardation, liver enlargement, skin lesions, dry and brittle hair showing trichorrhexis nodosa microscopically and fluorescing red, convulsions, and episodic unconsciousness.
  • Sequence similarities

    Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.
  • Post-translational
    modifications

    Acetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity.
  • Information by UniProt
  • Database links

  • Alternative names

    • Argininosuccinase antibody
    • Argininosuccinate lyase antibody
    • Arginosuccinase antibody
    • ARLY_HUMAN antibody
    • ASAL antibody
    • ASL antibody
    • EC 4.3.2.1 antibody
    see all

Images

  • All lanes : Anti-Argininosuccinate Lyase antibody (ab96215) at 1/1000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : Argininosuccinate Lyase Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 52 kDa

  • Anti-Argininosuccinate Lyase antibody (ab96215) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 52 kDa



    10% SDS PAGE

References

ab96215 has not yet been referenced specifically in any publications.

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