Key features and details
- Rabbit polyclonal to Argininosuccinate Lyase
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Argininosuccinate Lyase antibody
See all Argininosuccinate Lyase primary antibodies
DescriptionRabbit polyclonal to Argininosuccinate Lyase
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant fragment corresponding to Human Argininosuccinate Lyase aa 86-335.
Database link: P04424
- WB: MOLT-4, HeLa, IHC-P: DLD1 xenograft. ICC/IF: HeLa.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 52 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
PathwayAmino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.
Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-L-arginino)succinate: step 1/1.
Involvement in diseaseDefects in ASL are the cause of arginosuccinicaciduria (ARGINSA) [MIM:207900]. Arginosuccinicaciduria is an autosomal recessive disorder of the urea cycle. The disease is characterized by mental and physical retardation, liver enlargement, skin lesions, dry and brittle hair showing trichorrhexis nodosa microscopically and fluorescing red, convulsions, and episodic unconsciousness.
Sequence similaritiesBelongs to the lyase 1 family. Argininosuccinate lyase subfamily.
modificationsAcetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity.
- Information by UniProt
- Argininosuccinase antibody
- Argininosuccinate lyase antibody
- Arginosuccinase antibody
Paraffin embedded DLD1 xenograft stained for ASL using ab96215 at 1/500 dilution in immunohistochemical analysis.
HeLa cells stained for ASL (green) using ab96215 at 1/500 dilution in ICC/IF.
All lanes : Anti-Argininosuccinate Lyase antibody (ab96215) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell extract
Lane 2 : Argininosuccinate Lyase Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 52 kDa
Anti-Argininosuccinate Lyase antibody (ab96215) at 1/1000 dilution + MOLT-4 whole cell lysate at 30 µg
Predicted band size: 52 kDa
10% SDS PAGE
ab96215 has not yet been referenced specifically in any publications.