Overview

  • Product name

    Anti-Argininosuccinate Lyase antibody
    See all Argininosuccinate Lyase primary antibodies
  • Description

    Rabbit polyclonal to Argininosuccinate Lyase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 13 - 231 of Human Argininosuccinate Lyase (AAH08195).

  • Positive control

    • WB: 293T, A431, H1299, HeLa, HepG2 and MOLT4 cell lysates IF: HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Pathway

    Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.
    Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-L-arginino)succinate: step 1/1.
  • Involvement in disease

    Defects in ASL are the cause of arginosuccinicaciduria (ARGINSA) [MIM:207900]. Arginosuccinicaciduria is an autosomal recessive disorder of the urea cycle. The disease is characterized by mental and physical retardation, liver enlargement, skin lesions, dry and brittle hair showing trichorrhexis nodosa microscopically and fluorescing red, convulsions, and episodic unconsciousness.
  • Sequence similarities

    Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.
  • Post-translational
    modifications

    Acetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity.
  • Information by UniProt
  • Database links

  • Alternative names

    • Argininosuccinase antibody
    • Argininosuccinate lyase antibody
    • Arginosuccinase antibody
    • ARLY_HUMAN antibody
    • ASAL antibody
    • ASL antibody
    • EC 4.3.2.1 antibody
    see all

Images

  • All lanes : Anti-Argininosuccinate Lyase antibody (ab97370) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 52 kDa



    10% SDS PAGE
  • ab97370, at a 1/200 dilution, staining Argininosuccinate Lyase in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.

References

This product has been referenced in:

  • Baruteau J  et al. Argininosuccinic aciduria fosters neuronal nitrosative stress reversed by Asl gene transfer. Nat Commun 9:3505 (2018). Read more (PubMed: 30158522) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (A375)
Loading amount
20 µg
Specification
A375
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

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Verified customer

Submitted Jan 21 2011

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