Recombinant Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19396] to Argininosuccinate Lyase
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Argininosuccinate Lyase antibody [EPR19396]
See all Argininosuccinate Lyase primary antibodies -
Description
Rabbit monoclonal [EPR19396] to Argininosuccinate Lyase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver and testis lysates; HepG2, T-47D, A549, SH-SY5Y, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse and Rat liver lysates. IP: Human fetal liver whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19396 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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IP |
1/30.
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Notes |
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WB
1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
IP
1/30. |
Target
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Pathway
Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.
Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-L-arginino)succinate: step 1/1. -
Involvement in disease
Defects in ASL are the cause of arginosuccinicaciduria (ARGINSA) [MIM:207900]. Arginosuccinicaciduria is an autosomal recessive disorder of the urea cycle. The disease is characterized by mental and physical retardation, liver enlargement, skin lesions, dry and brittle hair showing trichorrhexis nodosa microscopically and fluorescing red, convulsions, and episodic unconsciousness. -
Sequence similarities
Belongs to the lyase 1 family. Argininosuccinate lyase subfamily. -
Post-translational
modificationsAcetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity. - Information by UniProt
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Database links
- Entrez Gene: 435 Human
- Entrez Gene: 109900 Mouse
- Entrez Gene: 59085 Rat
- Omim: 608310 Human
- SwissProt: P04424 Human
- SwissProt: Q91YI0 Mouse
- SwissProt: P20673 Rat
- Unigene: 632015 Human
see all -
Alternative names
- Argininosuccinase antibody
- Argininosuccinate lyase antibody
- Arginosuccinase antibody
see all
Images
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All lanes : Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026) at 1/5000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human testis tissue lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
Lane 5 : Mouse liver lysate
Lane 6 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 51 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 2 seconds; Lane 2: 1 minute; Lane 3 and 4: 5 seconds; Lane 5 and 6: 1 second.
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All lanes : Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026) at 1/2000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026) at 1/2000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Argininosuccinate Lyase antibody [EPR19396] (ab201026) at 1/5000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 2 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Argininosuccinate Lyase is predominantly expressed in the liver, and weakly expressed in the lung (PMID: 8576237).
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Argininosuccinate Lyase was immunoprecipitated from 0.35 mg of Human fetal liver whole cell lysate with ab201026 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab201026 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Human fetal liver whole cell lysate, 10µg (Input).
Lane 2: ab201026 IP in Human fetal liver whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab201026 in Human fetal liver whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab201026 has been referenced in 3 publications.
- Soria LR et al. Beclin-1-mediated activation of autophagy improves proximal and distal urea cycle disorders. EMBO Mol Med 13:e13158 (2021). PubMed: 33369168
- Chow JPH et al. A modified arginine-depleting enzyme NEI-01 inhibits growth of pancreatic cancer cells. PLoS One 15:e0231633 (2020). PubMed: 32353864
- Puleston DJ et al. Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation. Cell Metab 30:352-363.e8 (2019). PubMed: 31130465