Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ago2 / eIF2C2 antibody - ChIP Grade

Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Specification
brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Other product details

Dilution
1/2000
Incubation time
45 minute(s) · Temperature: 20°C · Diluent: Antibody Diluent (Dako)

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit HRP polymer (pre-diluted, Dako)
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Additional data

Additional Notes
Previous abreview positive for breast cancer - here we tried using brain. At higher concentrations the background was very high though at 1/200 cytoplasmic staining in some cells was observed (examples are arrowed). This staining was absent in the negative control.

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Submitted Apr 06 2009

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