Immunocytochemistry/ Immunofluorescence abreview for Anti-Ago2 / eIF2C2 antibody - ChIP Grade

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200

Additional data

Additional Notes
Asynchronous HeLa were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody appears to give a focal staining pattern that is slightly enriched within cytoplasm. Methanol fixation also appears to work well.

Dr. Kirk Mcmanus

Verified customer

Submitted May 28 2009

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