CLIP abreview for Anti-Ago2 / eIF2C2 antibody - ChIP Grade

Inconclusive
Abreviews
Application
CLIP
Sample
Human Cell lysate - whole cell (HEK 293T)
Specification
HEK 293T
Type
iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control
Anti-hnRNP C antibody (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control
Protein A dynabeads without antibody bound
Immuno-precipitation step
Other - Protein A dynabeads

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Autoradiogram Exposure Time: 16

Additional data

Additional Notes
UV crosslinking appears to be the limiting step in the iCLIP process, further optimisation of UV crosslinking may be needed before the effieciency of immunoprecipitation by this antibody can be assessed.

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Submitted Mar 21 2012

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