Immunocytochemistry/ Immunofluorescence abreview for Anti-Ago2 / eIF2C2 antibody - ChIP Grade

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (saos-2 human osteosarcoma cell line)
Specification
saos-2 human osteosarcoma cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS TRITON 0.25%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Other product details

Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Diluent: PBS-BSA 1%

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti rabbit ALEXA FLUOR 488 FROM INVITROGEN
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/250

Additional data

Additional Notes
blue: DAPI
green: Anti-Ago2 / eIF2C2

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Submitted Apr 19 2012

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