Western blot abreview for Anti-Ago2 / eIF2C2 antibody - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse nueroblastoma N2A cell)
Loading amount
30 µg
Specification
Mouse nueroblastoma N2A cell
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Li-Cor Goat anti rabbit IR800`
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Dilution
1/15000

Detection

Detection method
Li-Cor Odyssey Scanner
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: approx 100 kDa

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Submitted Jul 03 2012

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