Immunocytochemistry/ Immunofluorescence abreview for Anti-Argonaute-2 antibody - ChIP Grade

Below Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse muscle tissue)
Permeabilization
Yes - Triton X-100
Specification
mouse muscle tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1%BSA, 10 % GS in TBST
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa flour 488 anti rabbit secondary
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
On muscle tissue, the signal is pernuclear and around cell membrane, but high background staining by IF
Abcam response
We would like to thank the reviewer for submitting the AbReview and the honest feedback about the use of ab32381 in IHC-Fr. We have also received the information, that a previous batch of the antibody worked well on cryo sections, so the suitability for this application may be batch-dependent. Please be aware that the application is not covered with our AbPromise guarantee. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.

Nesrin Sabha

Verified customer

Submitted Feb 28 2018

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