Key features and details
- Rabbit polyclonal to Argonaute-2 (phospho Y393)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Argonaute-2 (phospho Y393) antibody
See all Argonaute-2 primary antibodies
DescriptionRabbit polyclonal to Argonaute-2 (phospho Y393)
SpecificityThe site is conserved in Argonaute-1 and Argonaute-4, but not in Argonaute-3.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Fish
Synthetic peptide corresponding to Human Argonaute-2 (phospho Y393) conjugated to keyhole limpet haemocyanin.
Database link: Q9UKV8
- A431 cell lysate treated with 100 ng/mL EGF for 60 min.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 49% PBS, 0.1% BSA, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab215746 was cross-absorbed to unphospho-Argonaute-2 (phospho Y393), before affinity purification using Argonaute-2 (phospho Y393) peptide (without carrier).
Our Abpromise guarantee covers the use of ab215746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).|
FunctionRequired for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
Sequence similaritiesBelongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.
DomainThe Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However while RNase H utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of Asp-Asp-His (DDH).
modificationsHydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity.
Cellular localizationCytoplasm > P-body. Nucleus. Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8.
- Information by UniProt
- Ago 2 antibody
- AGO2_HUMAN antibody
- Argonaute 2 antibody
Lanes 1-2 : rat monoclonal anti-Ago2
Lanes 3-4 : Anti-Argonaute-2 (phospho Y393) antibody (ab215746) at 1/1000 dilution
Lane 1 : A431 cells treated with EGF (100 ng/ml for 60 min.)
Lanes 2 & 4 : A431 cells treated with EGF (100 ng/ml for 60 min.) treated with lambda phosphatase
Lane 3 : A431 cell lysate treated with EGF (100 ng/ml for 60 min.)
Predicted band size: 97 kDa
Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, and 0.04% Tween20 for 1 h at room temperature.
ab215746 has been referenced in 1 publication.
- Liu T et al. AGO2 phosphorylation by c-Src kinase promotes tumorigenesis. Neoplasia 22:129-141 (2020). PubMed: 31981897