Anti-AGO3 antibody (ab3593)
Key features and details
- Rabbit polyclonal to AGO3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-AGO3 antibody
See all AGO3 primary antibodies -
Description
Rabbit polyclonal to AGO3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
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ICC/IF | (1) |
1/200.
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Notes |
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WB
1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). |
ICC/IF
1/200. |
Target
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Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. -
Sequence similarities
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Cellular localization
Cytoplasm > P-body. - Information by UniProt
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Database links
- Entrez Gene: 192669 Human
- Entrez Gene: 214150 Mouse
- Entrez Gene: 313593 Rat
- GenBank: NP_079128 Human
- GenBank: NP_803171 Human
- GenBank: NP_700451 Mouse
- Omim: 607355 Human
- SwissProt: Q9H9G7 Human
see all -
Alternative names
- 5730550L01Rik antibody
- Ago 3 antibody
- Ago3 antibody
see all
Images
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All lanes : Anti-AGO3 antibody (ab3593) at 2 µg/ml
Lane 1 : Dorsal Root Ganglia (Rat) Tissue Lysate
Lane 2 : Dorsal Root Ganglia (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands. -
Rat dorsal root ganglia were fixed in paraformaldehyde, blocked with 2% BSA for 30 minutes then incubated with ab3593 (1/200 dilution). For further details please refer to Hengst U et al (2006).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab3593 has been referenced in 1 publication.
- Hengst U et al. Functional and selective RNA interference in developing axons and growth cones. J Neurosci 26:5727-32 (2006). PubMed: 16723529