Anti-AGO4 antibody (ab85077)
Key features and details
- Rabbit polyclonal to AGO4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AGO4 antibody
See all AGO4 primary antibodies -
Description
Rabbit polyclonal to AGO4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human AGO4 (N terminal).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/250 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
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Notes |
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WB
1/250 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). |
Target
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Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV). -
Sequence similarities
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Cellular localization
Cytoplasm > P-body. - Information by UniProt
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Database links
- Entrez Gene: 192670 Human
- Entrez Gene: 76850 Mouse
- Entrez Gene: 298533 Rat
- Omim: 607356 Human
- SwissProt: Q9HCK5 Human
- SwissProt: Q8CJF8 Mouse
- Unigene: 471492 Human
- Unigene: 86705 Mouse
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Alternative names
- 5730550L01Rik antibody
- Ago 4 antibody
- Ago4 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab85077 has been referenced in 3 publications.
- Yu D et al. Multiple microRNAs function as self-protective modules in acetaminophen-induced hepatotoxicity in humans. Arch Toxicol 92:845-858 (2018). EMSA ; Human . PubMed: 29067470
- Voutila J et al. Development and Mechanism of Small Activating RNA Targeting CEBPA, a Novel Therapeutic in Clinical Trials for Liver Cancer. Mol Ther 25:2705-2714 (2017). PubMed: 28882451
- Zhong F et al. A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes. Nucleic Acids Res 43:10474-91 (2015). WB . PubMed: 26405199