Product nameAnti-Argonaute 5 antibody
DescriptionRabbit polyclonal to Argonaute 5
Specificityab4723 detects a band of ~ 75 kDa in P19 embryonic carcinoma lysate. The predicted molecular weight of murine Argonaute 5 is 72 kDa according to Swissprot entry Q8CGT7. (This represents a partial sequence) Other bands of 85, 160 and 250 kDa are also detected which may be non-specific binding or alternatively may correspond to the full length Argonaute 5.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to Mouse Argonaute 5 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in F9 whole cell lysate.
General notesSwiss prot Q8CGT7 - fragment
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab4723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).|
RelevanceArgonaute proteins make up a highly conserved family whose members have been implicated in RNAi and related phenomena in several organisms. In addition to roles in RNAi-like mechanisms, Argonaute proteins influence development, and at least a subset are involved in stem cell fate determination. Argonaute proteins are ~100-kD highly basic proteins that contain two common domains, namely PAZ and PIWI domains (Cerutti et al. 2000). The PAZ domain, consisting of 130 amino acids, has been identified in Argonaute proteins and in Dicer (Bernstein et al. 2001a). Although it has no defined function, PAZ is thought to be a protein-protein interaction domain, potentially mediating both homo- and heterodimerization (Cerutti et al. 2000). There are several reports that Argonaute proteins, being highly basic, might bind RNA. Miwi, a murine homolog of Piwi, exists in a complex with its target mRNAs in vivo (Deng and Lin 2002). Furthermore, Drosophila Ago1 has been shown to bind ribohomopolymers in vitro (Kataoka et al. 2001). Of course, the involvement of Argonaute proteins in RNAi and related phenomena presupposes an interaction with RNA, either directly or indirectly. However, detailed studies of Argonaute structure and biochemistry will be required to reveal its precise function in RNA metabolism.
- Ago 5 antibody
- Ago5 antibody
- Argonaute 5 protein antibody
30µg of p19 murine embryonic carcinoma cell lysate was run on an SDS-PAGE and incubated for 12 hours with ab4723 (1/1000 dilution). A specific band of ~ 75 kDa is detected.
Anti-Argonaute 5 antibody (ab4723) at 1 µg/ml + F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab4723 has not yet been referenced specifically in any publications.