Western blot abreview for Anti-Argonaute 5 antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (P19 embryonic carcinoma cell line)
Loading amount
30 µg
Specification
P19 embryonic carcinoma cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Polyclonal Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
15 second(s)
Bands
Specific: 75 kDa Non-specific: 85, 160, 250 kDa
Positive control
mouse p19 cell lysate

Additional data

Abcam response
Thank you for your review of ab4273. The predicted molecular weight of murine Argonaute 5 is ~ 72 kDa according to Swissprot entry Q8CGT7 therefore it is likely the band that is detected at ~ 75 kDa corresponds to Argonaute 5. At this time we have no explanation for the bands at 85, 160 and 250 kDa. The swissprot entry refers to a partial sequence, so it is possible that the 85 kDa band represents full length Argonaute 5, alternatively these three bands may be a result of non-specific binding.

Ms.

Verified customer

Submitted May 26 2006

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