Thank you for your review of ab4273. The predicted molecular weight of murine Argonaute 5 is ~ 72 kDa according to Swissprot entry Q8CGT7 therefore it is likely the band that is detected at ~ 75 kDa corresponds to Argonaute 5. At this time we have no explanation for the bands at 85, 160 and 250 kDa. The swissprot entry refers to a partial sequence, so it is possible that the 85 kDa band represents full length Argonaute 5, alternatively these three bands may be a result of non-specific binding.
Submitted May 26 2006
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