Overview

  • Product name

    Anti-ARH antibody [EPR13115] - N-terminal
    See all ARH primary antibodies
  • Description

    Rabbit monoclonal [EPR13115] to ARH - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ARH aa 1-100. The exact sequence is proprietary.
    Database link: Q5SW96

  • Positive control

    • Humab fetal liver, K562 and 293T cell lysates. K562 cells; mouse heart and kidney and rat heart and kidney tissue lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 34 kDa.
IP 1/10 - 1/100.

Target

  • Function

    Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface.
  • Tissue specificity

    Expressed at high levels in the kidney, liver, and placenta, with lower levels detectable in brain, heart, muscle, colon, spleen, intestine, lung, and leukocytes.
  • Involvement in disease

    Defects in LDLRAP1 are the cause of autosomal recessive hypercholesterolemia (ARH) [MIM:603813]. ARH is a disorder caused by defective internalization of LDL receptors (LDLR) in the liver. ARH has the clinical features of familial hypercholesterolemia (FH) [MIM:143890] homozygotes, including severely elevated plasma LDL cholesterol, tuberous and tendon xanthomata, and premature atherosclerosis. LDL receptor (LDLR) activity measured in skin fibroblasts is normal, as the LDL binding ability.
  • Sequence similarities

    Contains 1 PID domain.
  • Domain

    The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction the AP-2 complex subunit AP2B1.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARH antibody
    • ARH GENE antibody
    • ARH_HUMAN antibody
    • ARH1 antibody
    • ARH2 antibody
    • Autosomal recessive hypercholesterolemia protein antibody
    • DKFZp586D0624 antibody
    • FHCB1 antibody
    • FHCB2 antibody
    • LDL receptor adaptor protein antibody
    • Ldlrap1 antibody
    • Low density lipoprotein receptor adapter protein 1 antibody
    • MGC34705 antibody
    • OTTHUMP00000008526 antibody
    see all

Images

  • All lanes : Anti-ARH antibody [EPR13115] - N-terminal (ab181043) at 1/1000 dilution

    Lane 1 : Mouse heart tissue lysates
    Lane 2 : Mouse kidney tissue lysates
    Lane 3 : Rat heart tissue lysates
    Lane 4 : Rat kidney tissue lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

  • Western blot analysis of ARH in immunoprecipitation pellets from K562. ab181043 used at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

  • Anti-ARH antibody [EPR13115] - N-terminal (ab181043) at 1/1000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1000 µg

    Predicted band size: 34 kDa

  • All lanes : Anti-ARH antibody [EPR13115] - N-terminal (ab181043) at 1/5000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : 293T cell lysatae

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Rodríguez-Jiménez C  et al. A new variant (c.1A>G) in LDLRAP1 causing autosomal recessive hypercholesterolemia: Characterization of the defect and response to PCSK9 inhibition. Atherosclerosis N/A:N/A (2019). Read more (PubMed: 30777337) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - other (Huh 7.5 liver cells)
Gel Running Conditions
Reduced Denaturing (12% polyacrilamyde)
Loading amount
15 µg
Treatment
siRNA treated cells
Specification
Huh 7.5 liver cells
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Sep 29 2015

Application
Western blot
Sample
Human Cell lysate - other (Huh 7.5 liver cells)
Gel Running Conditions
Reduced Denaturing (12% polyacrilamyde)
Loading amount
15 µg
Treatment
siRNA treated cells
Specification
Huh 7.5 liver cells
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Aug 31 2015

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