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RecombinantRabMAb

Recombinant Anti-ARH antibody [EPR13116] - BSA and Azide free (ab250127)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-ARH antibody [EPR13116] - BSA and Azide free (ab250127)
  • Anti-ARH antibody [EPR13116] - BSA and Azide free (ab250127)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13116] to ARH - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

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Conjugation
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HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
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Recombinant Human ARH protein (ab123174)

View more associated products

Overview

  • Product name

    Anti-ARH antibody [EPR13116] - BSA and Azide free
    See all ARH primary antibodies
  • Description

    Rabbit monoclonal [EPR13116] to ARH - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250127 is the carrier-free version of ab179828 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab250127 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13116
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-ARH antibody [EPR13116] - C-terminal (ab179828)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human ARH protein (ab123174)
  • Related Products

    • Recombinant Human ARH protein (ab123174)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface.
    • Tissue specificity

      Expressed at high levels in the kidney, liver, and placenta, with lower levels detectable in brain, heart, muscle, colon, spleen, intestine, lung, and leukocytes.
    • Involvement in disease

      Defects in LDLRAP1 are the cause of autosomal recessive hypercholesterolemia (ARH) [MIM:603813]. ARH is a disorder caused by defective internalization of LDL receptors (LDLR) in the liver. ARH has the clinical features of familial hypercholesterolemia (FH) [MIM:143890] homozygotes, including severely elevated plasma LDL cholesterol, tuberous and tendon xanthomata, and premature atherosclerosis. LDL receptor (LDLR) activity measured in skin fibroblasts is normal, as the LDL binding ability.
    • Sequence similarities

      Contains 1 PID domain.
    • Domain

      The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction the AP-2 complex subunit AP2B1.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession Q5SW96 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 26119 Human
      • Omim: 605747 Human
      • SwissProt: Q5SW96 Human
      • Unigene: 590911 Human
      • Alternative names

        • ARH antibody
        • ARH GENE antibody
        • ARH_HUMAN antibody
        • ARH1 antibody
        • ARH2 antibody
        • Autosomal recessive hypercholesterolemia protein antibody
        • DKFZp586D0624 antibody
        • FHCB1 antibody
        • FHCB2 antibody
        • LDL receptor adaptor protein antibody
        • Ldlrap1 antibody
        • Low density lipoprotein receptor adapter protein 1 antibody
        • MGC34705 antibody
        • OTTHUMP00000008526 antibody
        see all

      Images

      • Western blot - Anti-ARH antibody [EPR13116] - BSA and Azide free (ab250127)
        Western blot - Anti-ARH antibody [EPR13116] - BSA and Azide free (ab250127)
        All lanes : Anti-ARH antibody [EPR13116] - C-terminal (ab179828) at 1/10000 dilution

        Lane 1 : Human fetal liver lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : K562 cell lysate
        Lane 4 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 34 kDa



        This data was developed using ab179828, the same antibody clone in a different buffer formulation.

         

      • Anti-ARH antibody [EPR13116] - BSA and Azide free (ab250127)
        Anti-ARH antibody [EPR13116] - BSA and Azide free (ab250127)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab250127? Please let us know so that we can cite the reference in this datasheet.

      ab250127 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab250127.
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