Recombinant
RabMAb

Recombinant Anti-ARH antibody [EPR13116] - BSA and Azide free (ab250127)

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Overview

  • Product name

    Anti-ARH antibody [EPR13116] - BSA and Azide free
    See all ARH primary antibodies

  • Description

    Rabbit monoclonal [EPR13116] to ARH - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human ARH aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q5SW96

  • General notes

    ab250127 is the carrier-free version of ab179828 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab250127 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab250127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface.

    • Tissue specificity

      Expressed at high levels in the kidney, liver, and placenta, with lower levels detectable in brain, heart, muscle, colon, spleen, intestine, lung, and leukocytes.

    • Involvement in disease

      Defects in LDLRAP1 are the cause of autosomal recessive hypercholesterolemia (ARH) [MIM:603813]. ARH is a disorder caused by defective internalization of LDL receptors (LDLR) in the liver. ARH has the clinical features of familial hypercholesterolemia (FH) [MIM:143890] homozygotes, including severely elevated plasma LDL cholesterol, tuberous and tendon xanthomata, and premature atherosclerosis. LDL receptor (LDLR) activity measured in skin fibroblasts is normal, as the LDL binding ability.

    • Sequence similarities

      Contains 1 PID domain.

    • Domain

      The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction the AP-2 complex subunit AP2B1.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • ARH antibody
        • ARH GENE antibody
        • ARH_HUMAN antibody
        • ARH1 antibody
        • ARH2 antibody
        • Autosomal recessive hypercholesterolemia protein antibody
        • DKFZp586D0624 antibody
        • FHCB1 antibody
        • FHCB2 antibody
        • LDL receptor adaptor protein antibody
        • Ldlrap1 antibody
        • Low density lipoprotein receptor adapter protein 1 antibody
        • MGC34705 antibody
        • OTTHUMP00000008526 antibody
        see all

      References

      ab250127 has not yet been referenced specifically in any publications.

      Publishing research using ab250127? Please let us know so that we can cite the reference in this datasheet.

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