Anti-ARHGAP1 antibody (ab194425)
Key features and details
- Mouse polyclonal to ARHGAP1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ARHGAP1 antibody -
Description
Mouse polyclonal to ARHGAP1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment (GST-tag) corresponding to Human ARHGAP1 aa 300 to the C-terminus (C terminal). NCBI Accession No. AAH18118.
Database link: Q07960 -
Positive control
- MES-SA/Dx5, jurkat, mouse NIH3T3, mouse Raw 264.7 and rat PC12 cell lysates; recombinant ARHGAP1 protein fragment.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2500. Predicted molecular weight: 50 kDa.
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Notes |
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WB
1/500 - 1/2500. Predicted molecular weight: 50 kDa. |
Target
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Function
GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 CRAL-TRIO domain.
Contains 1 Rho-GAP domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 392 Human
- Entrez Gene: 228359 Mouse
- Entrez Gene: 311193 Rat
- Omim: 602732 Human
- SwissProt: Q07960 Human
- SwissProt: Q5FWK3 Mouse
- Unigene: 138860 Human
- Unigene: 22413 Mouse
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Alternative names
- ARHGAP 1 antibody
- Arhgap1 antibody
- CDC42 GTPase activating protein antibody
see all
Images
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Anti-ARHGAP1 antibody (ab194425) at 1/1000 dilution + recombinant ARHGAP1 protein fragment at 0.2 µg
Developed using the ECL technique.
Predicted band size: 50 kDa -
Anti-ARHGAP1 antibody (ab194425) at 1/500 dilution + rat PC12 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa -
Anti-ARHGAP1 antibody (ab194425) at 1/500 dilution + MES-SA/Dx5 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa -
Anti-ARHGAP1 antibody (ab194425) at 1/500 dilution + mouse Raw 264.7 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa -
Anti-ARHGAP1 antibody (ab194425) at 1/500 dilution + Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa -
Anti-ARHGAP1 antibody (ab194425) at 1/500 dilution + mouse NIH 3T3 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab194425 has been referenced in 1 publication.
- Li JP et al. ARHGAP1 overexpression inhibits proliferation, migration and invasion of C-33A and SiHa cell lines. Onco Targets Ther 10:691-701 (2017). WB ; Human . PubMed: 28223826