Product nameAnti-ARHGAP1 antibody - N-terminal
See all ARHGAP1 primary antibodies
DescriptionRabbit polyclonal to ARHGAP1 - N-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Rhesus monkey
Recombinant fragment within Human ARHGAP1 (N terminal). The exact sequence is proprietary.
Database link: Q07960
- ICC/IF: HeLa cells. WB: ARHGAP1-transfected HEK-293T lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 50 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionGTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.
Sequence similaritiesContains 1 CRAL-TRIO domain.
Contains 1 Rho-GAP domain.
- Information by UniProt
- ARHGAP 1 antibody
- Arhgap1 antibody
- CDC42 GTPase activating protein antibody
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ARHGAP1 (green) using ab228678 (1/500 dilution) in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
All lanes : Anti-ARHGAP1 antibody - N-terminal (ab228678) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : ARHGAP1-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Developed using the ECL technique.
Predicted band size: 50 kDa
ab228678 has not yet been referenced specifically in any publications.