Key features and details
- Rabbit polyclonal to ARHGAP1 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARHGAP1 antibody - N-terminal
See all ARHGAP1 primary antibodies
DescriptionRabbit polyclonal to ARHGAP1 - N-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Rhesus monkey
Recombinant fragment within Human ARHGAP1 (N terminal). The exact sequence is proprietary.
Database link: Q07960
- ICC/IF: HeLa cells. WB: ARHGAP1-transfected HEK-293T lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 50 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionGTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.
Sequence similaritiesContains 1 CRAL-TRIO domain.
Contains 1 Rho-GAP domain.
- Information by UniProt
- ARHGAP 1 antibody
- Arhgap1 antibody
- CDC42 GTPase activating protein antibody
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ARHGAP1 (green) using ab228678 (1/500 dilution) in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
All lanes : Anti-ARHGAP1 antibody - N-terminal (ab228678) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : ARHGAP1-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Developed using the ECL technique.
Predicted band size: 50 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228678 has not yet been referenced specifically in any publications.