Overview

  • Product name

    Anti-ARHGAP1 antibody - N-terminal
    See all ARHGAP1 primary antibodies
  • Description

    Rabbit polyclonal to ARHGAP1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human ARHGAP1 (N terminal). The exact sequence is proprietary.
    Database link: Q07960

  • Positive control

    • ICC/IF: HeLa cells. WB: ARHGAP1-transfected HEK-293T lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab228678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 1 CRAL-TRIO domain.
    Contains 1 Rho-GAP domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARHGAP 1 antibody
    • Arhgap1 antibody
    • CDC42 GTPase activating protein antibody
    • Cdc42 GTPase-activating protein antibody
    • CDC42GAP antibody
    • GTPase activating protein rhoOGAP antibody
    • GTPase-activating protein rhoOGAP antibody
    • GTPase-activating protein, Rho, 1 antibody
    • OTTHUMP00000233485 antibody
    • OTTHUMP00000233486 antibody
    • p50 RhoGAP antibody
    • p50-RhoGAP antibody
    • p50rhoGAP antibody
    • RHG01_HUMAN antibody
    • Rho GTPase activating protein 1 antibody
    • Rho GTPase-activating protein 1 antibody
    • Rho related small GTPase protein activator antibody
    • Rho type GTPase activating protein 1 antibody
    • Rho-related small GTPase protein activator antibody
    • Rho-type GTPase-activating protein 1 antibody
    • RHOGAP antibody
    • RHOGAP1 antibody
    see all

Images

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ARHGAP1 (green) using ab228678 (1/500 dilution) in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

  • All lanes : Anti-ARHGAP1 antibody - N-terminal (ab228678) at 1/500 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
    Lane 2 : ARHGAP1-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    12% SDS-PAGE

References

ab228678 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228678.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up