Key features and details
- Rabbit polyclonal to ARHGAP10
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARHGAP10 antibody
DescriptionRabbit polyclonal to ARHGAP10
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human ARHGAP10 aa 542-744.
LMRPQEETVAALMDLKFQNIVVEILIENHEKIFRTPPDTTFPEPTCLSAS PPNAPPRQSKRQGQRTKRPVAVYNLCLELEDGDNPYPSKEDTPTSSLDSL SSPSPVTTAVPGPPGPDKNHLLADGGSFGDWASTIPGQTRSSMVQWLNPQ SPTTTSSNSAVTPLSPGSSPFPFSPPATVADKPPESIRSRKARAVYPCEA EHS
Database link: A1A4S6
- IHC-P: Human lung cancer tissue. ICC/IF: HepG2 (human liver hepatocellular carcinoma cell line) cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
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Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab222805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionGTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death.
Tissue specificityHigh levels of expression in heart and skeletal muscle.
Sequence similaritiesContains 1 BAR domain.
Contains 1 PH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain.
modificationsPhosphorylated. Phosphorylated in vitro by constitutive active PKN3.
Cellular localizationCytoplasm. Cytoplasm > perinuclear region. Cell membrane. Association to cell membrane is dependent on PH domain.
- Information by UniProt
- Arhgap10 antibody
- Graf-related protein 2 antibody
- GRAF2 antibody
Paraffin-embedded human lung cancer tissue stained for ARHGAP10 using ab222805 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ARHGAP10 (green) using ab222805 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
ab222805 has not yet been referenced specifically in any publications.