Anti-ARHGAP12 antibody (ab241475)

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Overview

  • Product name

    Anti-ARHGAP12 antibody

  • Description

    Rabbit polyclonal to ARHGAP12

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human ARHGAP12 aa 100-150. The exact sequence is proprietary. (NP_060757.4).
    Database link: Q8IWW6

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab241475 was affinity purified using an epitope specific to ARHGAP12 immobilized on solid support.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 96 kDa.
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Western blot - Anti-ARHGAP12 antibody (ab241475)
      Western blot - Anti-ARHGAP12 antibody (ab241475)
      All lanes : Anti-ARHGAP12 antibody (ab241475) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
      Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 96 kDa


      Exposure time: 30 seconds


      Lysates prepared using NETN lysis buffer.

    References

    ab241475 has not yet been referenced specifically in any publications.

    Publishing research using ab241475? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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