Anti-ARHGAP12 antibody (ab241475)
Key features and details
- Rabbit polyclonal to ARHGAP12
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ARHGAP12 antibody -
Description
Rabbit polyclonal to ARHGAP12 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human ARHGAP12 aa 100-150. The exact sequence is proprietary. (NP_060757.4).
Database link: Q8IWW6 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab241475 was affinity purified using an epitope specific to ARHGAP12 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab241475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/2000 - 1/10000. Predicted molecular weight: 96 kDa. |
Target
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Function
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. -
Sequence similarities
Contains 1 PH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain.
Contains 2 WW domains. - Information by UniProt
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Database links
- Entrez Gene: 94134 Human
- Entrez Gene: 75415 Mouse
- Omim: 610577 Human
- SwissProt: Q8IWW6 Human
- SwissProt: Q8C0D4 Mouse
- Unigene: 499264 Human
- Unigene: 239675 Mouse
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Alternative names
- Arhgap12 antibody
- FLJ10971 antibody
- FLJ20737 antibody
see all
Images
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All lanes : Anti-ARHGAP12 antibody (ab241475) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 96 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241475 has not yet been referenced specifically in any publications.