Overview

  • Product name

    Anti-ARHGAP17 antibody - N-terminal
    See all ARHGAP17 primary antibodies
  • Description

    Rabbit polyclonal to ARHGAP17 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ARHGAP17 aa 41-70 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q68EM7

  • Positive control

    • IHC-P: Human placenta tissue. WB: K562 cell lysate. Flow Cytometry: K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 95 kDa.
IHC-P 1/100.
Flow Cyt 1/10 - 1/50.

Target

  • Relevance

    ARHGAP17 is a Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. It specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. RHGAP17 participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1.
  • Cellular localization

    Cytoplasm; Cell membrane (tight junction)
  • Database links

  • Alternative names

    • MST066 antibody
    • MST110 antibody
    • MSTP038 antibody
    • MSTP066 antibody
    • MSTP110 antibody
    • NADRIN antibody
    • Neuron associated developmentally regulated protein antibody
    • PP367 antibody
    • PP4534 antibody
    • Rho GTPase activating protein 17 antibody
    • Rho type GTPase activating protein 17 antibody
    • RhoGAP interacting with CIP4 homologs 1 antibody
    • RhoGAP interacting with CIP4 homologs protein 1 antibody
    • RICH 1 antibody
    • RICH1 antibody
    • RICH1B antibody
    • WBP15 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human placenta tissue stained for ARHGAP17 with ab230889 at 1/100 dilution in immunohistochemical analysis.

  • Anti-ARHGAP17 antibody - N-terminal (ab230889) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 95 kDa

  • Flow Cytometry analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells, labeling ARHGAP17 with ab230889 at a 1/10 dilution (green) compared to a negative control (blue).

    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab230889 has not yet been referenced specifically in any publications.

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