Key features and details
- Rabbit polyclonal to ARHGAP17 - N-terminal
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARHGAP17 antibody - N-terminal
See all ARHGAP17 primary antibodies
DescriptionRabbit polyclonal to ARHGAP17 - N-terminal
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human ARHGAP17 aa 41-70 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q68EM7
- IHC-P: Human placenta tissue. WB: K562 cell lysate. Flow Cytometry: K562 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 95 kDa.|
|Flow Cyt||1/10 - 1/50.|
RelevanceARHGAP17 is a Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. It specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. RHGAP17 participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1.
Cellular localizationCytoplasm; Cell membrane (tight junction)
- MST066 antibody
- MST110 antibody
- MSTP038 antibody
Formalin-fixed, paraffin-embedded human placenta tissue stained for ARHGAP17 with ab230889 at 1/100 dilution in immunohistochemical analysis.
Anti-ARHGAP17 antibody - N-terminal (ab230889) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 95 kDa
Flow Cytometry analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells, labeling ARHGAP17 with ab230889 at a 1/10 dilution (green) compared to a negative control (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230889 has not yet been referenced specifically in any publications.