Overview

  • Product name
  • Description
    Rabbit polyclonal to ARHGAP18
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ARHGAP18 aa 186-216 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8N392

  • Positive control
    • MDA-MB231 cell lysate, Human breast carcinoma tissue, MDA-MB231 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 75 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
  • Sequence similarities
    Contains 1 Rho-GAP domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4833419J07Rik antibody
    • AI314906 antibody
    • AI843501 antibody
    • Arhgap18 antibody
    • bA307O14.2 antibody
    • FLJ25728 antibody
    • MacGAP antibody
    • MGC126757 antibody
    • MGC138145 antibody
    • OTTHUMP00000017190 antibody
    • OTTMUSP00000028606 antibody
    • RHG18_HUMAN antibody
    • Rho GTPase-activating protein 18 antibody
    • Rho-type GTPase-activating protein 18 antibody
    • SENEX antibody
    see all

Images

  • Anti-ARHGAP18 antibody (ab175970) at 1/1000 dilution + MDA-MB231 cell lysate at 35 µg

    Predicted band size: 75 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling ARHGAP18 using ab175970 at a 1/50 dilution, followed by peroxidase-conjugation to the secondary antibody and DAB staining.

  • Flow cytometry analysis of MDA-MB231 cells labeling ARHGAP18 using ab175970 at a 1/10 dilution (bottom histogram), compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175970 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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