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Synthetic peptide within Human ARHGAP18 aa 186-216 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q8N392
Our Abpromise guarantee covers the use of ab175970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 75 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling ARHGAP18 using ab175970 at a 1/50 dilution, followed by peroxidase-conjugation to the secondary antibody and DAB staining.
Flow cytometry analysis of MDA-MB231 cells labeling ARHGAP18 using ab175970 at a 1/10 dilution (bottom histogram), compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab175970 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"