Overview

  • Product name

    Anti-ARHGAP22 antibody
  • Description

    Rabbit polyclonal to ARHGAP22
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human ARHGAP22 aa 493-560.
    Sequence:

    DNVPAPGLVPGIPSVASMAWSGASSSESSVGGSLSSCTACRASDSSARSS LHTDWALEPSPLPSSSED


    Database link: Q7Z5H3

  • Positive control

    • RT-4 cell lysate; Human testis tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, 59% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab204743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250. Predicted molecular weight: 77 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. Acts as a GTPase activator for the RAC1 by converting it to an inactive GDP-bound state. Inhibits RAC1-dependent lamellipodia formation. May also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter.
  • Sequence similarities

    Contains 1 PH domain.
    Contains 1 Rho-GAP domain.
  • Cellular localization

    Cytoplasm. Nucleus. Mainly cytoplasmic. Some fraction is nuclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARHGAP22 antibody
    • RHG22_HUMAN antibody
    • Rho GTPase activating protein 2 antibody
    • Rho GTPase activating protein 22 antibody
    • Rho GTPase-activating protein 22 antibody
    • Rho type GTPase activating protein 22 antibody
    • Rho-type GTPase-activating protein 22 antibody
    • RhoGAP2 antibody
    • RhoGap22 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ARHGAP22  with ab204743 at 1/50 dilution.

  • Anti-ARHGAP22 antibody (ab204743) at 1/100 dilution + RT-4 cell lysate

    Predicted band size: 77 kDa

References

ab204743 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
100 µg
Specification
Liver
Blocking step
Odyssey as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100%

Abcam user community

Verified customer

Submitted Dec 28 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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