Overview

  • Product name

    Anti-ARHGAP23 antibody
  • Description

    Rabbit polyclonal to ARHGAP23
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: MNLGFGDESP EPEASGRGER LGRKVAPLAT TEDSLASIPF IDEPTSPSID LQAKHVPASA VVSSAMNSAP VLGTSPSS, corresponding to amino acids 509-586 of Human ARHGAP23.

  • Positive control

    • Human cerebellum tissue; RT 4 and U 251 MG lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab121617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function

    GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
  • Tissue specificity

    Expressed in placenta, prostate, hippocampus and brain medulla. Also expressed in brain tumor, salivary gland tumor, head and neck tumor.
  • Sequence similarities

    Contains 1 PDZ (DHR) domain.
    Contains 1 PH domain.
    Contains 1 Rho-GAP domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Arhgap23 antibody
    • KIAA1501 antibody
    • RHG23_HUMAN antibody
    • Rho GTPase-activating protein 23 antibody
    • Rho-type GTPase-activating protein 23 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and plasma membrane. Recommended concentration of ab121617 1-4 µg/ml. Cells treated with PFA/Triton X-100.

References

ab121617 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat Hippocampal Neurons)
Permeabilization
Yes - 0.2% Triton-X 100 in PBS
Specification
Rat Hippocampal Neurons
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 13 2017

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