Overview

  • Product name

    Anti-ARHGAP25 antibody [EPR13233-60]
    See all ARHGAP25 primary antibodies
  • Description

    Rabbit monoclonal [EPR13233-60] to ARHGAP25
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ARHGAP25 aa 450-600. The exact sequence is proprietary.
    Database link: P42331

  • Positive control

    • Ramos, Raji and Jurkat cell lysates; Human tonsil tissue lysate; Human stomach and hepatocellular carcinoma tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13233-60
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 77 kDa (predicted molecular weight: 73 kDa).

Target

Images

  • All lanes : Anti-ARHGAP25 antibody [EPR13233-60] (ab192020) at 1/1000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 73 kDa
    Observed band size: 77 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-ARHGAP25 antibody [EPR13233-60] (ab192020) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Human tonsil tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 73 kDa
    Observed band size: 77 kDa why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling ARHGAP25 using ab192020 at 1/200 dilution (5 μg/mL). A prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counterstain: Hematoxylin. Inset image: negative control obtained using PBS instead of ab192020.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling ARHGAP25 using ab192020 at 1/200 dilution (5 μg/mL). A prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counterstain: Hematoxylin. Inset image: negative control obtained using PBS instead of ab192020.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab192020 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab192020.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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