Overview

  • Product name

    Anti-ARHGAP25 antibody [EPR13233]
    See all ARHGAP25 primary antibodies
  • Description

    Rabbit monoclonal [EPR13233] to ARHGAP25
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ARHGAP25 aa 450-600. The exact sequence is proprietary.
    Database link: P42331

  • Positive control

    • Jurkat, Ramos and Daudi cell lysates; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13233
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 76 kDa (predicted molecular weight: 73 kDa).
IP 1/30 - 1/50.
ICC/IF 1/50 - 1/100.

Target

  • Function

    GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
  • Sequence similarities

    Contains 1 PH domain.
    Contains 1 Rho-GAP domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARHGAP 25 antibody
    • ARHGAP25 antibody
    • Epididymis secretory protein Li 308 antibody
    • HEL S 308 antibody
    • KAIA0053 antibody
    • RHG25_HUMAN antibody
    • Rho GTPase activating protein 25 antibody
    • Rho GTPase-activating protein 25 antibody
    • Rho type GTPase activating protein 25 antibody
    • Rho-type GTPase-activating protein 25 antibody
    see all

Images

  • All lanes : Anti-ARHGAP25 antibody [EPR13233] (ab181202) at 1/10000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 73 kDa

  • Immunofluorescent analysis of Jurkat cells (-20°C Acetone-fixed) labeling ARHGAP25 with ab181202 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Anti-ARHGAP25 antibody [EPR13233] (ab181202) at 1/10000 dilution + Ramos cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 73 kDa

  • Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab181202 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

References

ab181202 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181202.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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