Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13233] to ARHGAP25
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
Product nameAnti-ARHGAP25 antibody [EPR13233]
See all ARHGAP25 primary antibodies
DescriptionRabbit monoclonal [EPR13233] to ARHGAP25
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human ARHGAP25 aa 450-600. The exact sequence is proprietary.
Database link: P42331
- Jurkat, Ramos and Daudi cell lysates; Jurkat cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 76 kDa (predicted molecular weight: 73 kDa).|
|IP||1/30 - 1/50.|
|ICC/IF||1/50 - 1/100.|
FunctionGTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
Sequence similaritiesContains 1 PH domain.
Contains 1 Rho-GAP domain.
- Information by UniProt
- ARHGAP 25 antibody
- ARHGAP25 antibody
- Epididymis secretory protein Li 308 antibody
All lanes : Anti-ARHGAP25 antibody [EPR13233] (ab181202) at 1/10000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 73 kDa
Immunofluorescent analysis of Jurkat cells (-20°C Acetone-fixed) labeling ARHGAP25 with ab181202 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Anti-ARHGAP25 antibody [EPR13233] (ab181202) at 1/10000 dilution + Ramos cell lysate at 10 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 73 kDa
Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab181202 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
ab181202 has not yet been referenced specifically in any publications.