Key features and details
- Rabbit polyclonal to ARHGAP29
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ARHGAP29 antibody
See all ARHGAP29 primary antibodies
DescriptionRabbit polyclonal to ARHGAP29
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human ARHGAP29 aa 1211-1261.
Database link: NP_004806.3
- HeLa, 293T and NIH3T3 whole cell lysates.
This product was previously labelled as Rho GTPase activating protein 29
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85853 was affinity purified using an epitope specific to ARHGAP29 immobilized on solid support.
Our Abpromise guarantee covers the use of ab85853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 142 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionGTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho. In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis.
Tissue specificityWidely expressed. Highly expressed in skeletal muscle and heart. Expressed at intermediate level in placenta, liver and pancreas. Weakly expressed in brain, lung and kidney.
Sequence similaritiesContains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Rho-GAP domain.
- Information by UniProt
- ARHGAP 29 antibody
- Arhgap29 antibody
- ARHGAP29 protein antibody
All lanes : Anti-ARHGAP29 antibody (ab85853) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 142 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Detection of ARHGAP29 by Western Blot of Immunprecipitate.
ab85853, at 1 µg/ml, staining ARHGAP29 in HeLa whole cell lysate immunoprecipitated using ab85853 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab85853 has not yet been referenced specifically in any publications.