Product nameAnti-ARHGAP4 antibody
See all ARHGAP4 primary antibodies
DescriptionRabbit polyclonal to ARHGAP4
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
Synthetic peptide within Human ARHGAP4 aa 700-750. The exact sequence is proprietary.
Database link: P98171
- WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226462 was affinity purified using an epitope specific to ARHGAP4 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 105 kDa.|
RelevanceARHGAP4 (Rho GTPase-activating protein 4) has an inhibitory effect on stress fiber organization. It is a member of the group of signaling proteins involved in regulation of the small GTP-binding proteins of the RAS superfamily.
- ARHGAP 4 antibody
- C1 antibody
- GTPase-activating protein, Rho, 4 antibody
All lanes : Anti-ARHGAP4 antibody (ab226462) at 0.1 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 3 minutes
Lysate prepared using NETN lysis buffer.
ARHGAP4 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226462 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226462 at 0.4 µg/ml.
Lane 1: ab226462 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
ab226462 has not yet been referenced specifically in any publications.