Anti-ARHGAP4 antibody (ab226835)
Key features and details
- Rabbit polyclonal to ARHGAP4
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARHGAP4 antibody
See all ARHGAP4 primary antibodies -
Description
Rabbit polyclonal to ARHGAP4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan -
Immunogen
Synthetic peptide within Human ARHGAP4 aa 350-400. The exact sequence is proprietary.
Database link: P98171 -
Positive control
- WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226835 was affinity purified using an epitope specific to ARHGAP4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 105 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 105 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Relevance
ARHGAP4 (Rho GTPase-activating protein 4) has an inhibitory effect on stress fiber organization. It is a member of the group of signaling proteins involved in regulation of the small GTP-binding proteins of the RAS superfamily. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 473833 Chimpanzee
- Entrez Gene: 100124430 Cow
- Entrez Gene: 102129586 Cynomolgus monkey
- Entrez Gene: 492245 Dog
- Entrez Gene: 100057993 Horse
- Entrez Gene: 393 Human
- Entrez Gene: 100442277 Orangutan
- Entrez Gene: 100523659 Pig
see all -
Alternative names
- ARHGAP 4 antibody
- C1 antibody
- GTPase-activating protein, Rho, 4 antibody
see all
Images
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All lanes : Anti-ARHGAP4 antibody (ab226835) at 0.1 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 30 secondsLysates were prepared using NETN lysis buffer.
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ARHGAP4 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226835 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226835 at 0.4 µg/ml.
Lane 1: ab226835 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226835 has not yet been referenced specifically in any publications.