Product nameAnti-ARHGAP4 antibody
See all ARHGAP4 primary antibodies
DescriptionRabbit polyclonal to ARHGAP4
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
Synthetic peptide within Human ARHGAP4 aa 350-400. The exact sequence is proprietary.
Database link: P98171
- WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226835 was affinity purified using an epitope specific to ARHGAP4 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 105 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceARHGAP4 (Rho GTPase-activating protein 4) has an inhibitory effect on stress fiber organization. It is a member of the group of signaling proteins involved in regulation of the small GTP-binding proteins of the RAS superfamily.
- ARHGAP 4 antibody
- C1 antibody
- GTPase-activating protein, Rho, 4 antibody
All lanes : Anti-ARHGAP4 antibody (ab226835) at 0.1 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 30 seconds
Lysates were prepared using NETN lysis buffer.
ARHGAP4 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226835 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226835 at 0.4 µg/ml.
Lane 1: ab226835 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab226835 has not yet been referenced specifically in any publications.