Overview

  • Product name
  • Description
    Goat polyclonal to ARHGEF14
  • Host species
    Goat
  • Specificity
    ab175441 is expected to recognize both reported isoforms (NP_001106203.1; NP_079255.3).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human ARHGEF14 aa 315-324 (internal sequence) (Cysteine residue). NP_001106203.1; NP_079255.3.
    Sequence:

    C_QLDNQATVQR


    Database link: O15068

  • Positive control
    • Jurkat and Human thymus lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab175441 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 128 kDa.

Target

  • Function
    Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42.
  • Sequence similarities
    Belongs to the MCF2 family.
    Contains 1 CRAL-TRIO domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
    Contains 1 spectrin repeat.
  • Domain
    The CRAL-TRIO domain is involved in interaction with inositol phospholipids.
    The DH domain is involved in interaction with CCPG1.
  • Cellular localization
    Cytoplasm. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGEF 14 antibody
    • DBL''s big sister antibody
    • DBS antibody
    • FLJ12122 antibody
    • Guanine nucleotide exchange factor DBS antibody
    • KIAA0362 antibody
    • MCF 2L antibody
    • MCF.2 cell line derived transforming sequence like antibody
    • MCF.2 transforming sequence-like antibody
    • MCF2 like protein antibody
    • MCF2 transforming sequence like protein antibody
    • MCF2-transforming sequence-like protein antibody
    • Mcf2l antibody
    • MCF2L_HUMAN antibody
    • OST antibody
    • OST oncogene antibody
    see all

Images

  • All lanes : Anti-ARHGEF14 antibody (ab175441) at 1 µg/ml

    Lane 1 : Jurkat lysates
    Lane 2 : Human thymus lysates

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 128 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.

References

ab175441 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175441.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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