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Goat polyclonal to ARHGEF14
ab175441 is expected to recognize both reported isoforms (NP_001106203.1; NP_079255.3).
Predicted to work with:
Mouse, Rat, Cow, Dog
Synthetic peptide corresponding to Human ARHGEF14 aa 315-324 (internal sequence) (Cysteine residue). NP_001106203.1; NP_079255.3.
Jurkat and Human thymus lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab175441 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 128 kDa.
Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42.
Belongs to the MCF2 family.
Contains 1 CRAL-TRIO domain. Contains 1 DH (DBL-homology) domain. Contains 1 PH domain. Contains 1 SH3 domain. Contains 1 spectrin repeat.
The CRAL-TRIO domain is involved in interaction with inositol phospholipids.
The DH domain is involved in interaction with CCPG1.
Cytoplasm. Cell membrane.
Information by UniProt
ARHGEF 14 antibody
DBL''s big sister antibody
Western blot - Anti-ARHGEF14 antibody (ab175441)
All lanes :
Anti-ARHGEF14 antibody (ab175441) at 1 µg/ml
Lane 1 :
Lane 2 :
Human thymus lysates
Lysates/proteins at 35 µg per lane.
Predicted band size:
Primary incubation was 1 hour. Detected by chemiluminescence.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"