Anti-ARHGEF3 antibody (ab224148)

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Overview

  • Product name

    Anti-ARHGEF3 antibody
    See all ARHGEF3 primary antibodies

  • Description

    Rabbit polyclonal to ARHGEF3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cynomolgus monkey, Orangutan

  • Immunogen

    Recombinant fragment corresponding to Human ARHGEF3 aa 427-497.
    Sequence:

    HSLQANDTFNKQQWLNCIRQAKETVLCAAGQAGVLDSEGSFLNPTTGSRE LQGETKLEQMDQSDSESDCSM


    Database link: Q9NR81-1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: RT4 and U-251 MG cell lysates. IHC-P: Human rectum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab224148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250. Predicted molecular weight: 37,60,61,64 kDa.

Target

  • Relevance

    Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. ARHGEF3 encodes a guanine nucleotide exchange factor, which specifically activates two members of the Rho GTPase family: RHOA and RHOB, both of which have a role in bone cell biology. It has been identified that genetic variation in ARHGEF3 plays a role in the determination of bone mineral density (BMD), suggesting a role in postmenopausal osteoporosis.

  • Cellular localization

    Cytoplasmic

  • Database links

    • Alternative names

      • 1200004I24Rik antibody
      • 59.8 kDA protein antibody
      • 9830169H03Rik antibody
      • ARHGEF 3 antibody
      • C76747 antibody
      • DKFZP434F2429 antibody
      • Exchange factor found in platelets and leukemic and neuronal tissues antibody
      • Exchange factor found in platelets and leukemic and neuronal tissues XPLN antibody
      • FLJ98126 antibody
      • GEF 3 antibody
      • GEF3 antibody
      • MGC118905 antibody
      • Rho guanine nucleotide exchange factor (GEF) 3 antibody
      • Rho guanine nucleotide exchange factor 3 antibody
      • RhoGEF protein antibody
      • STA 3 antibody
      • STA3 antibody
      • XPLN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGEF3 antibody (ab224148)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGEF3 antibody (ab224148)

      Paraffin-embedded human rectum tissue stained for ARHGEF3 using ab224148 at 1/500 dilution in immunohistochemical analysis.

    • Western blot - Anti-ARHGEF3 antibody (ab224148)
      Western blot - Anti-ARHGEF3 antibody (ab224148)
      All lanes : Anti-ARHGEF3 antibody (ab224148) at 1/100 dilution

      Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
      Lane 2 : U-251 MG (human brain glioma cell line) cell lysate

      Developed using the ECL technique.

      Predicted band size: 37,60,61,64 kDa

    References

    ab224148 has not yet been referenced specifically in any publications.

    Publishing research using ab224148? Please let us know so that we can cite the reference in this datasheet.

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